利用番茄为受体表达轮状病毒外壳蛋白VP7的研究  

Expression of VP7 of Rotavirus Outer Capsid Protein in Transgenic Tomato

作　　者：沈文涛[1] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,中国海南海口571101

出　　处：《生命科学研究》2004年第3期245-249,共5页Life Science Research

基　　金：国家自然科学基金资助项目(30060033)

摘　　要：将轮状病毒外壳蛋白VP7基因克隆到植物表达载体pBI121,并转化到根癌农杆菌(Agrobacteriumtumefaciens)菌株EHA105中,采用叶盘转化法转化番茄(lycopersiconesculentumMill.)栽培品种TX0014,获得了转基因植株.经PCR、PCR-Southernblot和Southernblot分析表明:VP7基因已整合到转基因番茄植株的核基因组中.RT-PCR、Westernblot分析表明:VP7蛋白在叶片和果实中均获得了表达.Cotyledons of tomato were transform ed with Agrobacterium Tumefaciens EHA105carrying the gene VP7coding rotavirus outer capsid prote in.After several times kanamycin-r esistant selection,the regenerate d tomato plantlets were analyzed further by PCR,PCR-Southern blot and Sou thern blot,the results confirmed that the VP7gene were integrated into the genome .The results of RT-PCR and Western bl ot of transgenic plants showed that VP7gene was expressed in leaves and frui ts of transgenic plants.

关 键 词：轮状病毒 VP7 番茄 遗传转化 

分 类 号：Q786[生物学—分子生物学]