心肌特异性新蛋白激酶p93和peroxiredoxin3的相互作用  

Interaction Between The Novel Cardiac-specific Protein Kinase p93 and Peroxiredoxin 3

作　　者：冯艳[1] 刘冬青[1] 王震[2] 曹慧青[1] 时娜[1] 邓仲端[3] 丁金凤[1] 孟宪敏[1] 

机构地区：[1]中国医学科学院中国协和医科大学阜外心血管病医院分子医学中心,北京100037 [2]华中科技大学同济医学院药理系,武汉430030 [3]华中科技大学同济医学院病理系,武汉430030

出　　处：《生物化学与生物物理进展》2004年第8期688-692,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 5 5 7;3 0 3 0 0 13 1);北京市重大科技资助项目 (H0 2 0 2 2 0 0 2 0 3 10 )~~

摘　　要：为了明确p93在心血管系统中的生物学功能及在信号传导通路中的作用 ,采用酵母双杂交技术 ,选择 p93N端第 10 1～ 372位氨基酸构建诱饵质粒 ,筛选成人心脏cDNA文库 ,寻找与之相互作用的蛋白质 ,结果得到蛋白peroxiredoxin 3(PRX 3) .采用体外结合实验和免疫共沉淀等方法 ,证实了原核和真核表达的 p93确实可与PRX3相互作用 ,通过双重荧光共定位 ,为二者的相互作用提供了定位依据 .研究提示p93可能参与心肌细胞中与PRX3有关的各种生理病理过程 ,如生长、分化、发育、凋亡。In an attempt to get insight into the biological functions of p93 in the cardiovascular system and its potential roles in the signaling pathway, a yeast two-hybrid technology was carried out to screen the adult heart cDNA library using the N-terminal fragment of p93 ( 101 similar to 372 aa) as bait. Peroxiredoxin 3 ( PRX 3) was identified as a novel p93 binding protein. Subsequently, the interaction was confirmed by in vitro binding assay and co-immunoprecipitation and p93 was showed to co-localize in part with PRX 3 in HEK-293FT cells by immuofluorescence. Taken together, these observations suggest that p93 may participate in various pathophysiologic processes mediated by PRX3 in cardiomyocyte, such as cell growth, differentiation, development, apoptosis and oxygen stress.

关 键 词：心肌特异性新蛋白激酶 p93 酵母双杂交 心肌细胞 生物学功能 信号传导通路 

分 类 号：Q55[生物学—生物化学]