幽门螺杆菌重组腺病毒双价疫苗的构建及免疫学评价  

Construction of Adenovirus-mediated Vaccine of Helicobacter pylori and Immune Test in Balb/c Mice

作　　者：刘秀丽[1] 刘纯杰[1] 陶好霞[1] 李淑琴[1] 李勣[1] 张兆山[1] 

机构地区：[1]北京生物工程研究所,北京100071

出　　处：《生物化学与生物物理进展》2004年第8期716-721,共6页Progress In Biochemistry and Biophysics

基　　金：国家高技术"863"计划资助项目 ( 2 0 0 1AA2 15 161)~~

摘　　要：幽门螺杆菌 (Hp)是导致胃炎、消化道溃疡和胃癌的的主要病原菌 .利用基因重组技术 ,成功将Hp的ureB和hspA基因融合 .将ureB hspA融合基因成功构建到腺病毒载体上 .检测结果表明 ,该重组腺病毒具有侵染真核细胞能力 ,且能在真核细胞中表达目标抗原 .以血清中IgG和新鲜粪便中sIgA ,评价其免疫效果 ,结果显示 。Helicobacter pylori is one of the most common infectious pathogenic agents in human being which causes gastritis, peptic ulcer disease, gastric adenocarcinoma and malignant tumour of the stomach. ure B and lisp A gene were cloned and fused into a shuttle vector pADTrack-CMV. The resultant plasmid was linarized by digesting with restriction endonuclease Pme I, and subsequently were contransformed into E. coli. BJ5183 with an adenoviral backbone plasmid PAdEasy-1. The linearized recombinant plasmid was transfected into adenovirus packaging cell lines HEK-293. The recombinant adenoviruses were typically generated within 7 to 12 days. The recombinant adenoviruses titer was monitored by GFP expression. ELISA was used to test whether adenoviruses efficiently express the Ure B-Hsp A antigen protein in HEK-293. To determine the immunogenicity of the recombinant adenovirus, the delayed type serum IgG, secreted IgA, in the immunized mice were tested. The results showed that both Immoral and mucusal immune response in mice were induced.

关 键 词：幽门螺杆菌 重组腺病毒 尿素酶B 热休克蛋白A 体液免疫 黏膜免疫反应 

分 类 号：R515[医药卫生—内科学] R392-33[医药卫生—临床医学]