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机构地区:[1]甘肃农业大学动物科学技术学院,甘肃兰州730070
出 处:《甘肃农业大学学报》2004年第4期374-377,共4页Journal of Gansu Agricultural University
基 金:国家科技攻关项目(2002BA901A29);教育部高校骨干教师资助计划([2000]65);甘肃省人事厅(0270909)资助
摘 要:采用不连续非变性聚丙烯酰胺凝胶电泳技术对42只岷县黑裘皮羊的血红蛋白(Hb)和转铁蛋白(Tf)基因座的多态性进行了研究。结果表明:岷县黑裘皮羊的Hb、Tf位点均存在多态性;Hb位点共检测到HbA、HbB和HbC 3个等位基因,共构成HbAA、HbBB、HbAC和HbBC 4种基因型,其中HbBB和HbB为优势基因型和优势基因;Tf位点共检测到TfBB、TfCC、TfAB、TfAC、TfAD、TfBC、TfCD 7种基因型,受TfA、TfB、TfC、TfD 4个复等位基因控制,其中TfCC和TfC为优势基因型和优势基因。The polymorphism of hemoglobin (Hb) and transferring(Tf) of 42 individuals of Minxian Black-fur sheep was detected by using polyacrylamide gel electrophoresis. The results indicated that both loci of Hb and Tf in population of Minxian Black-fur sheep had displayed certain polymorphism. Three alleles at the loci of Hb were found such as HbA, HbB and HbC that composed four genotypes as HbAA, HbBB, HbAC and HbBC, among which HbBB and HbB were the dominant gene type and gene respectively. Four alleles at the loci of Tf were found such as TfA, TfB, TfC and TfD under the controll of 7 genotypes as follow as TfBB, TfCC, TfAB, TfAC, TfAD, TfBC and TfCD, among which TfCC and TfC were the dominant gene type and gene respectively.
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