牡蛎鳃组织的培养及TBTCl对细胞活力的影响  

Tissue culture of oyster gills and the effects of TBTCl on cells activity

作　　者：邓瑞鹏[1] 韩雅莉[1] 敖丽梅[1] 李兴暖[1] 周小朋[1] 

机构地区：[1]汕头大学生物系,广东汕头515063

出　　处：《海洋环境科学》2004年第2期5-7,共3页Marine Environmental Science

基　　金：国家自然科学基金项目(30271033);广东省科技兴海重大项目(A200005F02)

摘　　要：采用无血清组织培养和台盼蓝排斥实验,从细胞形态、细胞成活率等方面分别研究了氯化三丁基锡(TributyltinChloride,TBTCl)对牡蛎鳃组织细胞的影响。实验结果表明,TBTCl对牡蛎鳃细胞的生长及活力均产生一定的影响,具有抑制牡蛎鳃细胞增生,造成牡蛎鳃细胞损伤以及致使鳃细胞死亡的作用。In this paper,the effect of TBT on the shape, activity and damage of culture oyster cells was studied. Gills from Saccostrea cucullata were cultured in vitro with RMPI 1640 medium in which where was not bovine calf serum. The cells activities exposured in different TBT concentrations for 5days were examined using the Trypan-blue experiment. The results showed that TBTCl has certain effects on the growth and activities of gill cells, such as the restrained growth of cells, the damage of cells and the death of cells.

关 键 词：僧帽牡蛎 鳃细胞 氯化三丁基锡 细胞培养 细胞活力 

分 类 号：S944.4[农业科学—水产养殖] X17[农业科学—水产科学]