螺旋藻多糖的提取分离纯化及结构初步研究  

Studied on the extraction and purification and structure of Spirulina maxima

作　　者：李亚清[1] 杨海波[2] 刘艳[2] 张淑芬[1] 

机构地区：[1]大连理工大学精细化工国家重点实验室,辽宁大连116012 [2]大连大学化学与化学工程系,辽宁大连116622

出　　处：《海洋环境科学》2004年第2期75-77,共3页Marine Environmental Science

基　　金：辽宁省博士科研启动基金资助项目(001059)

摘　　要：离心收集处于生长平衡后期的螺旋藻(Spirulinamaxima),通过渗透冲击协同冻融细胞破碎、冷水提取,TCA除蛋白、乙醇沉淀得到粗多糖。用SephadexG 50凝胶层析分离纯化,得到一种水溶性螺旋藻多糖。利用醋酸纤维薄膜电泳和SephadexG 100凝胶检验螺旋藻多糖纯度,证明该糖组分均一。根据红外光谱,元素分析的结果对该糖结构进行初步研究。Centrifuging and harvesting Spirulina maxima at the stationary phase, and fragmentizing cells by penetrated and frozen-melt, then extracted by cold water, removing proteins by TCA, at last, the raw polysaccharide was deposited by ethanol. A kind of water-dissolved Spirulina maxima polysaccharide was obtained by SephadexG-50 gel chromatography. The composition of this polysaccharide was uniformed by means of cellulose film electrophoresis and SephadexG-100 gel chromatography. The structure of this polysaccharide was the primarily study by means of IR analysis and element analysis.

关 键 词：螺旋藻多糖 分离纯化 结构分析 

分 类 号：Q53[生物学—生物化学] Q949