甲状旁腺激素对大鼠血清中转化生长因子-β_1的影响  

Effects of Parathyroid Hormone on the Expression of Transforming Growth Factor-β_1 in Rats

作　　者：吕国良[1] 张克勤[1] 唐伟[1] 刘翠萍[1] 覃又文[1] 刘超[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2004年第4期371-372,共2页Journal of Nanjing Medical University(Natural Sciences)

基　　金：135重点学科基金资助项目(苏卫科教2001;31号)

摘　　要：目的:研究去势及甲状旁腺激素间歇注射对大鼠转化生长因子-β1(TGF-13,)表达的影响。方法:3月龄雌性SD大鼠行卵巢切除术(OVX)或假手术(Sham),手术组于2周后分别皮下注射甲状腺激素1-34片段(FFH1-34)或溶剂,10天后处死;酶联免疫吸附法(ELISA)检测血清中TGF-β1含量。结果:雌性大鼠卵巢切除2周后血清中TGF-β1含量明显升高(P<0.05),甲状旁腺激素注射组与去势对照组无明显差异。结论:甲状旁腺激素短期注射并不影响血清中TGF-β1含量。To evaluate the effect of ovariectomy and intermittent injection of parathyroid hormone on Transforming growth factor PI (TGF-β1,) in rats. Methods:Three-month-old rats were ovarietomied(OVX) or sham-operated (SHAM). Two weeks later, OVX rats received daily subcutaneous injection of human parathyroid hormone fragment 1-34 (hPTH1-34), 6 μg/100 g body weight or vehicle for 10 d. Then all animals were sacrificed. Enzyme linked-immuno-sorbent assay (ELISA) was used to detect the TGF-fJi in serum. Results:There was an increased TGF-β1 concentration in OVX rat serum( P < 0. 05). However, no difference of TGF-β1 levels between the rats treated with and without hPTH1-34 was found. Conclusion: Our findings suggest that intermittent injection of PTH has no effect on the concentration of TGF-β1.

关 键 词：卵巢切除术 转化生长因子Β1 甲状旁腺激素 酶联免疫吸附 

分 类 号：R713.6[医药卫生—妇产科学]