烧伤大鼠血清对大鼠骨髓间充质干细胞基因表达的影响  

Effects of serum from burned rats on the gene expression in rat bone mesenchymal stem cells

作　　者：付小兵[1] 韩冰[2] 陈伟[1] 周岗[1] 孙同柱[1] 方利君[1] 白晓东[1] 

机构地区：[1]解放军第304医院,北京100037 [2]解放军第148医院

出　　处：《解放军医学杂志》2004年第7期574-576,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展规划 (编号G1 9990 542 0 4 );国家自然科学基金重点项目 (编号 30 2 30 370 );国家自然科学基金面上项目 (编号 30 1 70 966)资助课题

摘　　要：目的　观察烧伤大鼠血清对体外培养的大鼠骨髓间充质干细胞 (MSCs)基因表达的影响。方法　分离Wistar大鼠骨髓 ,原代培养MSCs,传代后分别用含有体积分数为 10 %的正常大鼠血清 (N组)和烧伤后 3天大鼠血清 (B组 )的F 12培养基培养 2 4h ,然后以含有 5 70 5种基因的寡核苷酸微阵列检测两组细胞基因表达的差异。结果　两组MSCs对比 ,有 4种基因表达存在明显差异。其中B组类固醇敏感基因 1(Ssg 1)表达减弱 ,而磷酸二羟基丙酮酰基转移酶 (Gnpat)基因、成纤维细胞生长因子 4 (FGF 4 )基因和与骨形成蛋白 2可诱导激酶的序列有部分相同的一条EST表达增强。结论　烧伤后大鼠血清刺激MSCs可使其基因表达发生改变 。Objective To investigate the effects of serum from burned rats on the gene expression in cultured rat mesenchymal stem cells (MSCs). Methods Bone marrow was extracted from sacrificed Wistar rats, and MSCs were then incubated for 24h in F-12 medium in the presence of normal rat serum (group N) or serum obtained from burned rats 3 days after injury (group B). Total RNA was extracted from both groups.The mRNA was isolated.An Oligo microarray containing 5705 genes was used to compare the differences of gene expression between two groups. Results There were four genes which expressed differently in two groups.In comparison with group N, the expression of steroid sensitive gene 1 was decreased, but that of fibroblast growth factor-4, dihydroxyacetonephosphate acyltransferase and a EST, which was moderately similar to Bmp2-inducible kinase, were increased in group B. Conclusion Serum from burned rats was able to change the gene expression of MSCs, and it might play the key role in wound repair.

关 键 词：间充质干细胞 基因表达 烧伤 血清 

分 类 号：R644[医药卫生—外科学]