高氧液体对兔窒息性中毒肺水肿指标的影响  

Protective effect of hyperoxic solution on phosgene-induced lung injury

作　　者：王玲[1] 柴伟[2] 徐礼鲜[3] 海春旭[4] 唐时荣[1] 魏树国 

机构地区：[1]解放军第150医院,洛阳471031 [2]第四军医大学唐都医院 [3]第四军医大学口腔医学院 [4]第四军医大学毒理教研室 [5]西安高氧医疗设备有限公司

出　　处：《解放军医学杂志》2004年第7期620-621,共2页Medical Journal of Chinese People's Liberation Army

基　　金：长江学者特配骨干教师计划资助课题;陕西省自然科学基金 (编号 99SM39)资助课题

摘　　要：目的　观察兔光气染毒后肺水肿指标及肺泡灌洗液成分的变化 ,并进一步探讨高氧液对光气中毒性损伤的保护作用。方法　 18只新西兰大白兔随机均分为正常对照组、平衡盐组 (光气中毒后输注平衡盐 30ml·kg-1)和高氧液组(光气中毒后输注高氧液 30ml·kg-1) ,各组动物均于实验开始后 8h处死 ,立即开胸取肺 ,称取全肺湿重和右肺湿重 ,测定肺湿干比 (W/D)、肺水含量 (LW)和肺系数 (L/B) ;左肺用 37℃生理盐水进行灌洗 ,每次 5ml,共 3次 ,总回收率达 90 %以上 ,测定支气管肺泡灌洗液 (BALF)中丙二醛 (MDA)含量、谷胱甘肽过氧化物酶(GSH Px)活性及蛋白含量。结果　与正常对照组比较 ,高氧液组、平衡盐组的肺水肿指标及BALF中MDA、蛋白含量均显著升高 (P <0 0 5或P <0 0 1) ,GSH Px活力明显下降 (P <0 0 5或P <0 0 1)。与平衡盐组比较 ,高氧液组GSH Px活力明显增高 (P <0 0 1) ,BALF中MDA、蛋白含量均显著下降 (P <0 0 5或P <0 0 1) ,W/D、LW和L/B明显降低 (P <0 0 5 )。结论　脂质过氧化参与了光气中毒性肺损伤的发病 。Objective To study the protective effect of a hyperoxic solution on phosgene-induced lung injury by observing the changes in W/D ratio, lung water (LW), and L/B, and MDA contents, GSH-PX activity, and protein contents in broncho-alveolar lavage fluid (BALF). Methods The rabbits were divided into normal control group, hyperoxic solution (HO) and balance salt(BS) groups.Group HO and Group BS inhaled phosgene, and hyperoxic solution was given intravenously in group HO, but BS was given in group BS. W/D, LW, L/B, and MDA contents,GSH-PX activity,protein contents in broncho-alveolar lavage fluid (BALF) were determined. Results The MDA contents, W/D, LW and L/B were increased, and GSH-PX activity was decreased significantly in Group HO and Group BS compared with control group (P<0.05 and P<0.01). The contents of MDA , W/D, LW and L/B of group HO were lower than those of group BS. GSH-PX acticity in group HO was higher than those of group BS (P<0.05 and P<0.01). Conclusion The results suggested that hyperoxic solution had preventive effects on lung injury induced by phosgene poisoning in rabbits.

关 键 词：光气 高氧液 肺损伤 丙二醛 支气管肺泡灌洗液 

分 类 号：R651.2[医药卫生—外科学]