p,p’-DDE对离体培养大鼠睾丸支持细胞转铁蛋白表达的影响  

The Effect of p,p'-DDE on Transferrin in Sertoli Cells of Rat Testis in Vitro

作　　者：刘宏凯[1] 王翀[1] 刘世海[1] 杨克敌[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《环境与健康杂志》2004年第4期207-209,共3页Journal of Environment and Health

基　　金：国家重点基础研究发展计划(973计划)项目基金资助(2002CB512908)

摘　　要：目的研究pp’-DDE对离体培养大鼠睾丸支持细胞转铁蛋白表达的影响。方法对大鼠睾丸支持细胞离体原代培养,运用一步法RT-PCR检测给与pp’-DDE后24h支持细胞转铁蛋白mRNA水平。结果睾丸支持细胞转铁蛋白mRNA水平随pp’-DDE所给剂量的增高而降低,且与对照组差异存在显著性(P<0.05),呈剂量依赖关系。结论pp’-DDE可抑制睾丸支持细胞转铁蛋白的基因转录,从而抑制其合成,导致精子发生障碍和生殖功能紊乱。Objective To explore the effect of p,p'-DDE on transferrin expression in Sertoli cells of rat testis in vitro. Methods The effects of p,p'-DDE on expression of mRNA of transferrin gene in primary cultured Sertoli cells exposed to p,p'-DDE for 24 h was examined by one-step RT-PCR methods. Results The transferrin mRNA levels were decreased with the increases of p,p'-DDE concentration in Sertoli cell cultures,and there was a significant dose-effect relationship(P<0.05). Conclusion p,p'-DDE could inhibit the translation of transferrin in rat Sertoli cells cultured in vitro.

关 键 词：杀虫药 有机氯 支持细胞 转铁蛋白 p p’-DDE 内分泌干扰物 

分 类 号：R994.6[医药卫生—毒理学]