纳米氧化锆强韧化高孔隙率磷酸钙人工骨细胞支架生物学评价  

Bio-compatibility of hyper-porosity Ca_3(PO_4)_2 artificial bone cells frame reinforced by NM ZrO_2 in vitro

作　　者：戴伯军[1] 李家顺[1] 贾连顺[1] 吕宏[1] 陈岗[1] 叶晓健[1] 蔡舒[2] 张立德[3] 

机构地区：[1]第二军医大学附属长征医院,上海凤阳路415号200003 [2]天津大学材料学院,天津300072 [3]中科院固体所,合肥230031

出　　处：《中国矫形外科杂志》2004年第13期998-1001,共4页Orthopedic Journal of China

基　　金：国家自然科学基金资助项目 (编号 :30 1 70 954)

摘　　要：目的 :对自行研制的纳米氧化锆强韧化高孔隙率磷酸钙人工成骨细胞支架 [hyper porosityCa3 (PO4) 2bonecellsframe/NMZrO2 reinforced以下简称支架 ]的体外生物相容性进行评价。方法 :采用细胞毒性试验、溶血试验和急性全身中毒试验 3种方法。结果 :( 1)培养的L92 9成骨细胞经支架浸提液处理后形态良好 ,增值旺盛 ,材料毒性评级为 0级 ;( 2 )溶血率为 2 2 3 % (小于 5 % ) ,符合溶血试验标准要求 ;( 3 )无急性全身中毒反应。结论 :支架复合物具有良好的生物相容性。Objective:To evaluate the bio-compatibility of hyper-porosity Ca 3(PO 4) 2 artificial bone cells frame reinforced by NM ZrO 2 in vitro.Method:The cytotoxicity,hemolysis and acute whole body toxicity were tested with standard method.Result:(1)There was no obvious change of attachment,morphology and proliferation after L929 mouse fibroblasts had been treated by extracts.The toxicity gradation of the material was 0,measured by a value of MTT assay;(2)Hemolysis ratio was 2.23%;(3)There was no obvious whole-body toxicity reaction.Conclusion:The hyper-porosity Ca 3(PO 4) 2 artificial bone cells frame reinforced by NM ZrO 2 is Bio-compatible to tissue cells.

关 键 词：纳米 氧化锆 磷酸钙 

分 类 号：R687[医药卫生—骨科学]