应用胶原壳聚糖体外构建组织工程心脏瓣膜的初步研究  

In vitro construction of tissue-engineered cardiac valve on collagen-chitosan scaffold

作　　者：赵东锷[1] 李若冰[1] 邱立成[1] 李玮[1] 刘维永[1] 汪钢[2] 朱金如[1] 

机构地区：[1]武警总医院心胸外科,北京100039 [2]第四军医大学西京医院心血管外科,西安710032

出　　处：《武警医学》2004年第6期414-416,共3页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金资助项目 ( 3 9970 73 3 )

摘　　要：目的　探讨应用胶原壳聚糖支架体外构建组织工程心脏瓣膜的可行性。方法　观察材料结构特点 ,并行兔皮下包埋实验 ,分别于 4、6、8、12周观察材料的生物相容性和降解率。将犬主动脉壁间质细胞和内皮细胞种植于壳聚糖胶原支架上 ,观察其形态并测定细胞合成胶原和前列环素的功能。结果　胶原壳聚糖膜呈网孔状结构 ,孔径 (10 3± 17) μm。皮下包埋实验显示材料生物相容性好 ,完全降解时间为 12周。种植实验示细胞在材料表面生长良好 ,并具有合成前列环素 (6 2 .3±3.2pg/mlvs 10 .7± 2 .1pg/ml,P <0 .0 5 )的功能。结论　以胶原壳聚糖膜为支架体外构建组织工程心脏瓣叶 ,种植细胞不仅能在材料表面生长 ,还能合成细胞间质和血管活性物质 。Objective To evaluate the feasibility of tissue-engineered cardiac valve on collagen-chitosan scaffold.Methods Structural characteristics of collagen-chitosan scaffold were observed by scanning electron microscopy. Collagen-chitosan membrane was implanted subcutaneously in rabbit and removed at weeks 4,6,8,and 12 to study its biocompatibility and biodegradability.Stromal and endothelial cells of canine aortic wall were seeded on the collagen-chitosan scaffold and their function of collagen and prostaglandin I2 synthesis was detected.Results The port diameter of collagen-chitosan scaffold was (103±17)μm.Collagen-chitosan scaffold had good biocompatibility and was completely degraded at the end of week 12.The cells implanted on the scaffold grew well and could synthesize PGI2(62.3±3.2 ng/L vs 10.7±2.1 ng/L,P<0.05).Conclusion It is feasible to construct tissue-engineered cardiac valve in vitro on collagen-chitosan scaffold.

关 键 词：胶原壳聚糖 组织工程 心脏瓣膜 生物相容性 降解率 

分 类 号：R318.12[医药卫生—生物医学工程]