大鼠附睾9型水通道表达  

Expressions of aquaporin 9 in capita and caudae epididymides of adult rats

作　　者：杨柯[1] 赵丹[1] 杨宝学[2] 王蔚东 赵雪俭[1] 徐桂香[4] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021 [2]美国加利福尼亚大学旧金山医学院心血管研究所医学生理学研究室,加利福尼亚旧金山ca94122 [3]丹麦Aarthus大学解剖学研究所细胞生物学研究室 [4]吉林省计划生育科研所IVF实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2004年第4期562-564,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题 (2 0 0 1110 )

摘　　要：目的 :观察大鼠附睾 9型水通道 (aquaporin9,AQP9)的表达。方法 :取 12周龄大鼠的附睾头部和尾部提取总 RNA和总蛋白进行 AQP9Northern blotting分析和 Western blotting分析。结果 :12周龄大鼠附睾存在 AQP9的表达 ,且附睾头部表达强于尾部。结论 :12周龄大鼠附睾头部 AQP9表达强于尾部 ,反映附睾不同部位对水的重吸收功能的差异。Objective To examine the expressions of aquaporin 9(AQP9) in various segments epididymides of adult rats. Methods The capita and caudae epididymides of 12 week rats were removed for total RNA extraction and total protein purification. Northern blotting analysis was performed with AQP9 cDNA probe, and Western blotting was performed with polyclonal anti-AQP9 antibody. Results The expressions of AQP9 were confirmed in rat epididymides and the AQP9 expression in capita epididymides was higher than that in caudae epididymides. Conclusion AQP9 expression is found in rat epididymides. The expression levels of AQP9 in rat capita and caudae epididymides show the reabsorption capacity in different segments of epididymides.

关 键 词：水蛋白类/代谢 附睾/代谢 免疫组织化学/方法 疾病模型 动物 

分 类 号：R339.21[医药卫生—人体生理学] R-332[医药卫生—基础医学]