不同强度的模拟推拉动作致大鼠海马神经元损伤的实验研究  

An Experimental Study on Injury of Hippocampus Neurons in Rats by Simulated Push-pull Maneuver of Different Degrees.

作　　者：郭素红[1] 王晓玉[1] 窦肇华[1] 姚萍[1] 张慧[1] 李云义[1] 

机构地区：[1]第四军医大学吉林军医学院,吉林吉林132013

出　　处：《航天医学与医学工程》2004年第2期98-102,共5页Space Medicine & Medical Engineering

基　　金：全军医药科研基金项目 ( 0 1MA2 0 2 )

摘　　要：目的探讨不同强度的推拉动作所致的推拉效应对大鼠海马神经元损伤的程度和时相性。方法应用动物模型评分、病理、电镜技术分别对不同强度模拟推拉动作后大鼠海马各区神经元损伤情况进行检测。结果 1 )动物模型评分 :随着推拉动作强度的加大 ,卒中指数和神经症状评分逐渐增高。 2 )HE染色 :对照组和± 2Gz暴露后各组及± 6Gz、± 8Gz暴露后的 30min组海马各区神经元形态及分布未见异常。± 6Gz、± 8Gz暴露后的 6h和 2 4h组可见轻重不等的神经元变性。 3)电镜超微结构检查 :± 2Gz暴露后 30min和 2 4h组 ,海马CA1区神经元与正常对照组比较无明显改变。± 2Gz暴露后 6h组及± 6Gz、± 8Gz暴露后的 6h和 2 4h实验组可见大鼠海马CA1区部分神经元出现程度不等的变性。结论± 6Gz和± 8Gz暴露后可引起大鼠海马神经元损伤 ,且以± 8Gz暴露后 6h损伤程度最重。Objective To explore the degree of injury and phase of push-pull effect induced by different degrees of push-pull maneuver on hippocampus neurons in rats. Method Injury of push-pull maneuver on hippocampus neurons in rats after different degrees of push-pull maneuver were determined by general evalution of animal model,pathologic examination and electronic microscopy.Result 1)General evalution of animal model: the apoplexy index and the pathlogical grading inceased with the increase of intensity of the push-pull maneuver.2)Routine HE :No abnormity was found in appearance and distribution of hippocampus neurons in the control rats and various groups of ±2 Gz exposures, and 30 min groups of ±6 Gz,±8 Gz exposures,but denatured necrosis of neurons were found in 6 h and 24 h groups of ±6 Gz ,±8 Gz exposures.3)Examination of ultrastructure under electronic microscope:No significant change were found in hippocampus neurons of CA1 area in 30 min and 24 h after±2 Gz exposures as compared with the normal control group .But various degrees of denatured necrosis of neurons were found in some of the hippocampus neurons of CA1 area in 6 h after ±2 Gz exposures, 6 h and 24 h after±6 Gz and ±8 Gz exposures. Conclusion ±6 Gz and ±8 Gz exposures may cause injury of hippocampus tissues and it is most severe at 6 h after the ±8 Gz exposures.

关 键 词：海马 神经元 推拉动作 推拉效应 损伤 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]