低氧习服对大鼠心肌总蛋白、丙二醛和一氧化氮含量的影响  

Effects of Hypoxic Acclimatization on Myocardial Contents of Total Protein,Malondialdehyde and Nitric Oxide in Rats

作　　者：龙超良[1] 尹昭云[2] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《航天医学与医学工程》2004年第2期114-116,共3页Space Medicine & Medical Engineering

基　　金：国家自然科学基金重点项目 ( 3 973 0 190 ) ;全军医药卫生科研基金重点课题 ( 0 1Z0 2 5 )

摘　　要：目的研究直接急性低氧和间断低氧习服后的急性低氧对大鼠心肌总蛋白、丙二醛 (malondialde hyde ,MDA)和一氧化氮 (nitricoxide ,NO)含量的影响。 方法经间断低氧习服 ( 30 0 0m、5 0 0 0m各 2周 ,4h/d)后的大鼠和正常大鼠经模拟海拔 80 0 0m缺氧 4h后 ,观察其心肌总蛋白、MDA和NO含量的变化。结果与常氧对照组相比 ,直接急性低氧组大鼠心肌总蛋白含量显著降低 ,MDA含量显著增高 ,NO代谢产物NO2 - 的含量显著降低 ,P值均 <0 .0 1 ;经过低氧习服后急性低氧与直接急性低氧组比较 ,大鼠心肌总蛋白含量显著增高 (P <0 .0 1 ) ,MDA含量显著降低 (P <0 .0 1 ) ,NO2 - 含量显著增高 (P <0 .0 5 )。结论大鼠经间断低氧习服后 ,比不习服时心肌总蛋白和NO2 - 含量显著增高 ,MDA含量显著降低 ,是心脏低氧习服的生化机制之一。Objective To study the effects of acute hypoxia and acute hypoxia after intermittent hypoxic acclimatization on myocardial contents of total protein, malondialdehyde(MDA) and nitric oxide(NO )in rats. Method Myocardial contents of total protein, MDA and NO were investigated in rats after intermittent hypoxic acclimatization (simulated high altitude 3 000 m and 5 000 m, 4 h/d, 2 weeks respectively) and normal rats after they were exposed to hypoxia (simulated high altitude 8 000 m) for 4 h. Result Compared with normal control rats, the myocardial contents of total protein and NO in acute hypoxic rats were reduced significantly, and the myocardial content of MDA was increased significantly. After intermittent hypoxic acclimatization, the myocardial contents of total protein and NO of rats were significantly higher and the myocardial content of MDA was significantly lower compared with those in acute hypoxic rats. Conclusion After intermittent hypoxic acclimatization, the myocardial contents of total protein and NO of rats were increased significantly and the content of myocardial MDA was decreased significantly. This is one of the biochemical mechanisms of hypoxic acclimatization of the heart.

关 键 词：高空缺氧 低氧习服 总蛋白 丙二醛 一氧化氮 

分 类 号：R852.11[医药卫生—航空、航天与航海医学] R854[医药卫生—临床医学]