氟致人胎肝细胞DNA损伤及其对细胞凋亡和p53蛋白表达的影响  

Effects on DNA damage and apoptosis and p53 protein expression induced by fluoride in human embryo hepatocytes

作　　者：哈建利[1] 褚启龙[1] 王爱国[2] 夏涛[2] 杨克敌[2] 

机构地区：[1]湖北职业技术学院,孝感432000 [2]华中科技大学同济医学院

出　　处：《卫生研究》2004年第4期400-402,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目 (No .30 2 71 1 55)

摘　　要：目的　研究氟对L 0 2细胞DNA的损伤作用及其对细胞凋亡和p5 3表达的影响 ,并探讨p5 3表达与细胞凋亡之间的关系。方法　体外培养的L 0 2细胞分别接触 0、4 0、80、1 6 0 μg ml氟化钠 (对照组、A、B、C组 ) 2 4h后 ,检测L 0 2细胞DNA损伤率、细胞凋亡百分率和p5 3蛋白表达水平。结果　染氟各组细胞DNA损伤率均明显高于对照组 (P <0 0 5 )。与对照组相比 ,B组和C组细胞凋亡百分率明显升高 (P <0 0 5 )。B组和C组细胞p5 3蛋白表达量均显著高于对照组 (P <0 0 1 )。结论　氟可导致L 0 2细胞DNA损伤率上升 ,诱导细胞凋亡和p5 3表达 ,并且随着氟浓度的升高 ,细胞凋亡率和p5Objective To study the effects of DNA damage and apoptosis and p53 expression, and to explore the relationship between apoptosis and p53 expression in human embryo hepatocytes induced by fluoride. Methods The rate of DNA damage, apoptosis and the level of p53 expression were investigated after the cells were incubated with sodium fluoride for about 24 hours. The concentrations of sodium fluoride of the control, A, B and C group were 0μg/ml, 40μg/ml, 80μg/ml and 160μg/ml individually. Results The rate of DNA damage of every group treated with sodium fluoride were significantly higher than the control group( P <0.05). The percentage of apoptosis of B and C groups increased apparently( P <0.05). The level of p53 expression of B and C groups were significantly higher than the control group( P <0.01). Conclusion Fluoride can increase the rate of DNA damage, and induce apoptosis and expression of p53 in human embryo hepatocytes. Furthermore, both apoptosis and the level of p53 expression, there exists a rise tendency with the increase concentration of fluoride.

关 键 词：氟 DNA损伤 细胞凋亡 P53 

分 类 号：Q813.7[生物学—生物工程] Q593.1