晕船适应大鼠下丘脑差减cDNA文库的构建  

Construction of the subtracted cDNA library in hypothalamus of the seasickness adaptive rats

作　　者：吉雁鸿[1] 郭俊生[1] 李敏[1] 赵法伋[1] 

机构地区：[1]第二军医大学军队卫生学教研室,上海200433

出　　处：《卫生研究》2004年第4期410-412,共3页Journal of Hygiene Research

摘　　要：目的　分离晕船适应大鼠下丘脑差异表达基因片段 ,以探讨晕船适应机理。方法　采用抑制消减杂交方法将经过模拟晕船刺激获得晕船适应大鼠与正常大鼠的下丘脑组织进行正、反向杂交 ,构建晕船适应大鼠下丘脑差减cDNA文库 ,应用dotblot进行阳性克隆的鉴定。结果　根据dotblot杂交结果送交测序 ,获得下丘脑差异表达基因片段 2 3条 ,上调 1 0条 ,5条为未知功能基因的部分序列 ,下调 1 3条 ,含 6条未知功能基因的部分序列。结论　分析部分含有相关EST的已知功能基因 ,提示SAM、包括加压素在内的神经内分泌系统。Objective To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.Methods Suppression subtract hybridization technique was used, and forward and reverse hybridization was performed on the hypothalamus of seasickness adaptive rats and that of normal rats so that to construct subtracted cDNA library. Dot blot was used for differential screening the subtracted library.Results 23 fragments of differentially expressed genes was obtained including 10 up regulating and 13 down regulating fragments.Conclusion Many played role in adaptability formation to seasickness such as SAM, vasopressin, and heme oxygenase.

关 键 词：晕船适应 大鼠 下丘脑 差异表达基因 

分 类 号：R87[医药卫生—运动医学] G804.7[医药卫生—临床医学]