脑室灌注β-淀粉样肽致痴呆动物模型  

The dementia models induced by intracerebroventricular infusion of β-Amyloid peptide in mice

作　　者：楚晋[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院北京老年病医疗研究中心药理研究室,北京100053

出　　处：《中国药理学通报》2004年第7期827-829,共3页Chinese Pharmacological Bulletin

基　　金：国家"973"重点基础研究发展计划项目;NoG2000057010;首都"248"重大创新工程项目;NoH010210130113;北京市自然科学基金项目;No7982006;北京市科委资助北京市重点科技实验室项目;No951890600;北京市中医药重点学科项目;No2001;首都医学发展基金项目;No200230

摘　　要：目的　建立脑室灌注 β 淀粉样肽 (Aβ)致痴呆动物模型及观测指标。方法　微泵渗透对动物脑室进行灌注可溶性大片段Aβ ,侧脑室一次性注射聚集态小片段Aβ,侧脑室一次性注射可溶性大片段Aβ片段 ,侧脑室注射Aβ联用转化生长因子β(TGFβ)。 结果　上述方法处理后动物水迷宫和被动回避操作能力受损 ,学习和记忆功能损害 ,皮层和海马中胆碱乙酰转移酶 (ChAT)活性明显降低 ,抗氧化能力下降 ,海马神经元坏死或缺失 ,凋亡相关蛋白酶Bal 2、Bax和p5 3增加等。结论　上述方法均可选用来作为模拟AD病理特征的动物模型。Aim To establish the dementia mice models induced by intracerebroventricular infusion of β-Amyloid peptide and related measuring index. Methods The mice models were made by intracerebroventricular infusion of long fragment of solubleβ-Amyloid peptide with micropump, intracerebroventricular injection of long solubleβ-Amyloid peptide fragment and short insoluble β-Amyloid peptide fragment,and intracerebroventricular injection of β-Amyloid peptide combining with transforming growth factor. Results Compared with normal control, the learning and memory ability was decreased in all above model mice. the ChAT activity in both hippocampus and cortex of model mice decreased, the hippocampal neuron were depleted and the content of apoptosis associated protein Bal-2?BAX and p53 were increased. Conclusions All above mice models could be choose as the dementia models which were simulating the pathological charactoristics of the Alzheimer's disease.

关 键 词：ALZHEIMER病 动物模型 Β-淀粉样肽 脑室 

分 类 号：R332[医药卫生—人体生理学] R322.81[医药卫生—基础医学]