氢溴酸东莨菪碱的含量和降解产物的测定  

Determination of scopolamine hydrobromide injection and its degradation product by ion-pair HPLC

作　　者：孔爱英[1] 陈新善[1] 张显杰[2] 

机构地区：[1]总后卫生部药品仪器检验所,北京100071 [2]中国人民解放军第309医院,北京100091

出　　处：《中国医院药学杂志》2004年第8期467-468,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :采用离子对色谱法测定氢溴酸东莨菪碱注射液的含量和降解产物。方法 :以ODS柱为固定相 ,0 .0 0 4 %磷酸溶液 (pH3.0 ) 乙腈 (5 0∶5 0 )配制的 0 .0 0 8mol·L-1十二烷基硫酸钠溶液为流动相 ,流速 1.0mL·min-1,用DAD检测器于波长2 10nm处测定。结果 :氢溴酸东莨菪碱在 5 .86 8～ 2 93.4mg·L-1范围内 ,与色谱峰峰面积呈良好线性关系 ,相关系数为0 .9999,平均加样回收率为 10 0 .7% ,RSD为 0 .8% (n =6 )。结论 :对氢溴酸东莨菪碱的含量及降解产物进行研究表明 ,方法简便、快速 ,结果准确。OBJECTIVE An ion-pair HPLC method was established for the determination of Scopolamine Hy drobromide injection and its degradation products.METHODS A C 18 column was used,the mobile phase was composed of 50 mL 0.004% phosphoric acid (pH 3.0) and 50 mL acetonitrile mixed with 0.008 mol·L -1 sodium dodecyl sulphate.The detection was performed at 210 nm at a flow rate of 1.0 mL·min -1.RESULTS The standard curve was linear in the range of 5.868～ 293.4 mg·L -1 with the excellent correlation coefficient of 0.9999.The average added rec overy was 100.7%,RSD was 0.8%(n=6).CONCLUSION This method is sensitive, accurate and reliable.

关 键 词：离子对色谱法 氢溴酸东莨菪碱 含量测定 

分 类 号：R972.2[医药卫生—药品]