生化过程工程与中药现代化  

Biochemical process engineerinmg and modernization of Chinese materia medica (Ⅰ)

作　　者：顾铭[1] 欧阳藩[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100080

出　　处：《中草药》2004年第8期841-844,共4页Chinese Traditional and Herbal Drugs

摘　　要：在生化工程 2 0余年的研究工作基础上 ,结合中药现代化开展了一些技术、材料与设备的研究开发工作。研究植物细胞、组织、器官大规模培养增殖技术 ,应用于细胞代谢产物、生物转化和人工育种及发根 ,实现了工厂化生产 ,并成功研制多种类型的生物反应器 ;在原有化学工程提取分离技术的基础上 ,发展了反应分耦合、微波辅助提取等新技术 ,实现了高效浸出 ,并且节能、节水 ;此外 ,又发展了包括反胶团萃取、一步三相萃取青霉素、泡沫分级分离、膜分离、高速逆流色谱分离纯化等新技术。高速逆流色谱技术是一种没有固相载体的液 -液多级逆流萃取技术 ,避免了不可逆吸附 ,已成功用于多种天然产物的分析和分离 ,作为研究中药指纹图谱的新方法具有很好的精密度和重视性 ,高效液相色谱、气相色谱、毛细管电脉、质谱等技术在指纹图谱研究中发挥了重要作用。与此同时 ,还研制了多种分离介质 ,并在药物的修饰与包埋方面做了大量工作。另外 ,在海洋药物研究领域 ,创造了连续培养连续采收的新流程、新技术 ,进行了转基因藻的培养 。Some studies corresponding modernization of Chinese materia m edica(CMM)were performed in the institute for the new technologies,materials,and facilities based on the achievements and experiences gotten in the past two dec ades.The technologies of large-scale propagation of plant cell,tissue,and organ were applied to the products of cell metabolism,biotransformation,and artificial breeding and rooting.Many new types of large-scale bioreactors were designed an d used not only for laboratory but also for manufactory.Reaction/separation inte gration technology,microwave-assisted extraction,and the other new extraction te chnologies were studied to realize high efficiency and low energy.Besides tradit ional separation and purification methods,new technologies,including extraction in reversed micelles,one step three-phase extraction on Penicillin,foam fraction ation,membrane separation,and high-speed counter-current chromatography(HSCC),we re developed.HSCCC is a kind of liquid-liquid partition chromatography without a ny solid matrix,which eliminates irreversible adsorption of samples on solid sup port in the conventional chromatographic column.It has been successfully applied to the analysis and separation of various natural products.At the same time,man y types of microsphere and microencapsules for controlled release and separation media were prepared and lots of attention was paid on drug modifying and embedd ing techniques.HSCCC was also applied as a new method to the study of CMM finger print and showed good precision and repeatability.High performance liquid chroma tography(HPLC),gas chromatography(GC),high performance capillary electrophoresis (HPCE),and mass spectrometer(MS)played a very important role in fingerprinting.C ontinuous propagation-continuous collection technological process was set for la rge-scale propagation of micro-algae in the study of marine drugs.Transgenic alg ae were cultured to prduce genetically engineered products.

关 键 词：植物细胞培养 分离纯化技术 中药指纹图谱 分离介质 药物修饰 药物包埋 海洋药物 

分 类 号：R917[医药卫生—药物分析学]