长江中下游地区菱属植物的DNA分子鉴别  

Identification of Trapa L. plants along middle-low reaches of Changjiang River by analyzing their DNA sequences

作　　者：保曙琳[1] 丁小余[1] 常俊[1] 沈洁[1] 唐凤[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097

出　　处：《中草药》2004年第8期926-930,共5页Chinese Traditional and Herbal Drugs

基　　金：江苏省教育厅自然基金资助项目 ( 0 1KJB180 0 0 5 )

摘　　要：目的　对野生菱和栽培菱种rDNAITS片段进行分析 ,探讨该片段在两大群体中的系统学及鉴别研究意义。方法　对菱的rDNAITS区进行了PCR扩增、测序 ,运用Clustal、Mega2 0等软件对ITS区进行序列分析鉴别。结果　获得rDNAITS1、ITS2和 5 8SrDNA完整序列 ,10个居群菱的ITS1与ITS2序列的长度分别为 2 34～2 36bp和 2 2 0～ 2 2 1bp ,5 8S区均为 16 4bp ,不同居群的碱基差异为 0 2 2 %～ 2 94 %。变异位点数为 16个 ,信息位点数 6个。用NJ法根据ITS序列数据构建系统发生树。结论　尽管菱属各居群间rDNAITS的差异百分率较小 ,其亲缘关系较近 ,但根据ITS序列的特征可以很好地鉴别野生菱、栽培菱 ,菱属植物有可能都起源于同一种野生类居群。Object To analyze the rDNA ITS sequences between wi ld plants and cultivars of Trapa L. and study the utility in p hylogenesis and identification of these two groups. Methods The ITS gene fragments were PCR amplified and sequenced. The rDNA ITS regions w ere analyzed by means of the software of Clustal and Mega 2.0. Result s The rDNA sequences of 234-236 bp ITS1, 220-221 bp ITS2 gene fragment , and 5.8 S rDNA for 164 bp evenly were obtained from ten populations of Trapa L. The intraspecific substitution varies from 0.22% to 2. 94%. The variable sites are 16 while informative sites are six. The phylogenet ic tree based on ITS data was set up by NJ method. Conclusion ITS sequence is a pretty good molecular marker which can identify wild plants of Trapa L. from their cultivars. Diversity of ITS in differen t populations is less at intraspecific level. It is infered that the plants of Trapa L. may be derived from the same population of one species .

关 键 词：菱属 RDNA ITS序列 种质鉴别 物种起源 

分 类 号：R282.710.3[医药卫生—中药学]