犬颅脑火器伤后早期AQP_4的表达及意义  

Expression and its significance of the AQP_4 during early period following craniocerebral missile wounds in canines

作　　者：武弋[1] 章翔[1] 费舟[1] 封亚平[1] 宋少军[1] 王彦刚[1] 贺晓生[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2004年第4期337-340,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：总后卫生部重点基金资助项目 (0 4LX0 37)

摘　　要：目的　探讨水通道蛋白 (AQP4)在犬颅脑火器伤后早期弹道旁的表达规律 ,及其与脑损伤程度的相关性。方法　在犬颅脑火器伤后不同时间点 (30min、1h、12h、2 4h)、不同部位即挫伤区和震荡区 (伤道旁 0 .5cm、5 .0cm处 ) ,以电镜观察超微结构变化情况 ,用干湿比重法测脑组织含水量 ,采用免疫组织化学染色法检测AQP4蛋白表达 ,并进行脑水肿的相关性分析。结果　伤后 30min即出现内皮细胞肿胀、紧密连接开放、细胞器变性、血管周围水肿等超微结构损伤征象 ,脑的含水量亦然 (P<0 .0 1) ,并随时间的推移逐渐加重。同时AQP4的表达迅速下降 (P <0 .0 1) ,12h达到最低 ,2 4h有所回升 ,与脑水肿的发生、发展呈负相关性 (r= 0 .932 ,P =0 .0 2 5 )。结论　火器伤后脑内AQP4早期的迅速下调 ,是机体对脑损伤所作出的一种保护性反应 ,推测其具有一定阻止血脑屏障破坏。Objective To investigate the regularity of aquaporin-4(AQP 4) expression at earlier period after craniocerebral missile wounds in canines, and the correlation with the cerebral lesions.Methods We samplinged at different periods (30 min、1 h、12 h、24 h)and different regions (brain contusion and brain concussion ) after wounding, recorded ultrastructural destruction under the electron microscope, surveyed the water contents by dry-wet measure, detected AQP 4 expression with immunohistochemistry staining, and analyzed the correlation to cerebral edema.Results 30 min after wounding, endothelial cell swelling, tight junction opening, organelle degeneration, and edema surrounding blood vessel could be obseved. Water content increased continuously( P <0.01). Decreasing in AQP 4 expression was obvious( P <0.01). The expression reached the minimum at 12 h, and was negatively correlative to cerebral edema( r =-0.932, P =0.025).Conclusion Decreasing in AQP 4 expression at earlier period after craniocerebral missile wounds is a protective response by corpse. That may be one of the functions to prevent blood brain barrier destruction and to relieve influences of the angioencephal- edema and cytotoxinencephal-edema.

关 键 词：犬 颅脑火器伤 基因表达 水通道蛋白 血脑屏障 脑水肿 

分 类 号：R651.15[医药卫生—外科学] R742.7[医药卫生—临床医学]