新型罗丹明类荧光标记探针的研究(Ⅰ)-氨基标记产物的质子化互变异构  

A Study on Novel Fluorescent Probe Containing Rhodamine Structure (Ⅰ)——Tautomerism and Spectroscopic Characteristics

作　　者：郁美娟[1] 孟庆华[1] 郑一宁[1] 张海凤[1] 曾圆[1] 

机构地区：[1]上海交通大学化学化工学院,上海200240

出　　处：《染料与染色》2004年第4期187-190,共4页Dyestuffs and Coloration

摘　　要：罗丹明B-N-琥珀酰亚胺酯可用于生命体系物质氨基标记的新型荧光探针。研究了标记产物在不同的pH介质中的紫外-可见及荧光光谱特性,结合质子化互变异构机理,对互变异构现象与光谱变化的相关性进行了考察,确认了仲酰胺在中性和碱性条件下形成无荧光性的螺环内酰胺结构。Rhodamine B succinimidyl ester is a novel amine reactive fluorescent probe. Both UV/Vis spectra and fluorescence spectra of the labeled compounds were studied in different pH media. The correlation between protonation, tautomerism and spectra properties was discussed. It was found that N-substituted rhodamine amides would readily change to non-fluorescent spirolactams in alkaline or neutral medium.

关 键 词：罗丹明B-N-琥珀酰亚胺酯 荧光探针 光谱特性 互变异构 

分 类 号：O657.3[理学—分析化学]