育亨宾对异丙酚麻醉中大鼠海马去甲基肾上腺素释放的影响  

Effects of propofol anesthesia on noradrenaline release in rat hippocampus

作　　者：贺赛琳[1] 张英民[1] 岳云[2] 陈小光[1] 郭徽[1] 刘苏[1] 

机构地区：[1]解放军304医院麻醉科,北京100037 [2]首都医科大学附属红十字朝阳医院麻醉科,北京100021

出　　处：《第四军医大学学报》2004年第15期1397-1399,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :观察大鼠异丙酚麻醉与中枢神经系统海马去甲基肾上腺素 (NA)的释放以及α2 NA受体抑制剂之间的相互关系 ,探讨中枢性NA机制在异丙酚麻醉中发生的作用 .方法 :选择 2 0只雄性SD大鼠 ,随机分为A ,B 2组 ,每组 1 0只 .A组 :微量泵以 1 0mg/(kg·h) ,6 0mg/(kg·h)的速度依次各静注 4 5min ,停止静注异丙酚直至动物清醒 .B组在输注异丙酚的同时腹腔内注射育亨宾 0 .5mg/kg ,余和A组相同 .采用微透析技术分别测定异丙酚麻醉前、中及苏醒期的大鼠海马区细胞外液的NA释放值 .结果 :A组和B组 ,NA的释放在整个麻醉过程中的变化趋势有显著差异 (P <0 .0 5 ) .其中B组的NA的释放量在整个麻醉过程中的变化波动较大并呈升高的趋势 ,最高为 (0 .1 9± 0 .0 2 ) μg/1 5min ,最低为 (0 .1 3± 0 .0 3) μg/1 5min ;而A组NA的释放量在整个麻醉过程中的变化则较平稳 ,最高为 (0 .1 6± 0 .0 1 ) μg/1 5min ,最低 (0 .1 4± 0 .0 2 ) μg/1 5min .结论 :异丙酚麻醉抑制海马区NA的释放 。AIM: To observe the influences of propofol anesthesia on the release of noradrenaline (NA)and α 2 NA receptor inhibitor yohimbine in rat hippocampus so as to identify the mechanisms of central noradrenaline in propofol anesthesia. METHODS: Twenty adult male SD rats were divided randomly into 2 groups. In group A, the rats were infused intravenously (IV) with propofol at a rate of 10 mg/(kg·h) for 45 min and 60 mg/(kg·h) for 45 min and in group B, the rats were given yohimbine 0.5 mg/kg intraperitoneally and were infused IV with propofol as did group A. The release of NA in the extra cellular liquid of hippocampus was quantified before propofol anesthesia, in propofol administration and after propofol administration by in vivo microdialysis technique. RESULTS: Compared with those of the group A, the NA levels in hippocampus of group B changed significantly during the whole anesthesia course, with the highest release being (0.19± 0.02) μg/15 min and the lowest release being (0.13±0.03) μg/15 min. The NA levels in group A changed steadily [from (0.16±0.01) μg/15 min to (0.14±0.02) μg/15 min], ( P <0.05). CONCLUSION: Propofol anesthesia can inhibit the NA release in hippocampus but yohimbine increases NA release in hippocampus in propofol anesthesia.

关 键 词：海马 脑 微透析 异丙酚 育亨宾 去甲基肾上腺素 大鼠 

分 类 号：R313[医药卫生—基础医学]