藏药7号水提液对大鼠离体胸主动脉条收缩作用的影响  

Effect of the Aqueous Extract of Tibetan Drug No.7 on the Contraction of Rat Isolated Thoracic Aortic Rings

作　　者：彭晓云[1] 陶燕铎[1] 温绍君[2] 于庆利[2] 王佐广[2] 梅丽娟[1] 王绿娅[2] 绍赟[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海810001 [2]北京心肺血管疾病研究所,北京安贞医院高血压研究室,北京100029

出　　处：《北京中医药大学学报》2004年第4期41-44,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：中国科学院生命科学与生物技术局"十五"预研项目 (No 2 10 2 3 4)

摘　　要：目的　通过观察藏药 7号水提液对大鼠离体胸动脉条收缩作用的影响 ,研究藏药 7号的降压机制。方法　以生理盐水作为对照组 ,观察藏药 7号水提液 (6g L)和维拉帕米 (Ver 0 0 13g L)对高K+ 液引起的主动脉条收缩的时效影响 ,观察对KCl、NE及CaCl2 引起的大鼠主动脉条收缩的量效曲线的影响 ,以及对NE引起的依赖于细胞内钙及细胞外钙收缩的影响。结果　藏药 7号水提液抑制高K+ 液引起的主动脉收缩 (P <0 0 0 1) ;而且可以使KCl、NE及CaCl2 引起的大鼠主动脉条收缩的量效曲线非平行右移 ,最大效应降低 ,呈非竞争性拮抗作用 (P <0 0 5 ) ;与维拉帕米相似 ,并且对NE引起的依赖于细胞内钙及细胞外钙的收缩均有抑制作用 (P <0 0 5 )。结论　提示藏药 7号的降压机制与钙离子通道拮抗剂一致 ,而且其作用效果比Ver平稳 ,其最大作用与Ver相近。Objective To observe the effect of the aqueous extract of Tibetan Drug No.7 (AETD No.7) on the contraction of rat isolated thoracic aortic rings in order to investigate the antihypertensive mechanism of AETD No.7. Method Normal saline was used as the control,the effects of AETD No.7 (6 g/L) and verapamil (0.013 g/L) on the time-effect characteristic of the contraction of the thoracic aortic rings induced by high concentration K + solution;on the dose-effect curves related with the contractions of thoracic aortic rings induced by KCl,NE and CaCl_2;and on the contractions dependent on both intercellular and intracellular Ca 2+ of thoracic aortic rings induced by NE,were observed. Results AETD No.7 could inhibit the contraction of the thoracic aortic rings induced by high concentration K + solution ( P <0.001),and cause the dose-effect curves related with the contractions of thoracic aortic rings induced by KCl,NE and CaCl_2 to shift to the right with an decrease in the maximum effective response,manifesting non-competitive antagonism ( P <0.05).Like verapamil,AETD No.7 also could inhibit the contractions dependent on both intercellular and intracellular Ca_ 2+ of thoracic aortic rings induced by NE ( P <0.05). Conclusion The experimental results suggest that the antihypertensive mechanism of AETD No.7 is in accord with that of calcium channel antagonist,and furthermore,the effect of AETD No.7 is more stable than that of verapamil,with a maximum of effect alike that of verapamil.

关 键 词：藏药7号水提液 大鼠离体主动脉条 收缩 维拉帕米 

分 类 号：R285.5[医药卫生—中药学]