用辅助性T细胞表位增强猪带绦虫DNA疫苗的免疫保护反应  

Enhancement of Immune Protection Responses of the Modified pCC27 DNA Vaccine Containing Helper T Lymphocyte Epitopes

作　　者：刁振宇[1] 邓小昭[1] 周宗安[1] 陶开华[1] 王元伦[1] 刘玉[1] 顾志香[1] 吴琳[1] 高键[1] 

机构地区：[1]南京军区军事医学研究所,江苏南京210002

出　　处：《药物生物技术》2004年第4期225-228,共4页Pharmaceutical Biotechnology

基　　金：江苏省"十五"攻关课题 苏科技 [2 0 0 0 ] 3 13号

摘　　要：在猪绦虫融合抗原 pCC2 7(本室从六钩蚴cDNA表达文库筛选的 3个保护性抗原经拼接所得 )基因片段 5′末端引入 1个通用型辅助性T淋巴细胞表位、2个谷胱甘肽还原酶的辅助性T淋巴细胞表位 (TGG) ,构建成DNA疫苗。通过肌肉注射途径将这种DNA疫苗免疫小鼠和仔猪。动物试验结果表明 ,TGG表位可提高小鼠血清 pCC2 7抗体IgG、IgG1、IgG2a的水平 ,且长时间保持较高应答水平。用该疫苗免疫仔猪获得了 89%的保护率。An universal helper T lymphocyte epitope and two GST e pitopes(TGG) were spliced to the 5end of pCC27 fusion gene to construct a DNA vaccine pVAX-STGG-CC27. Mice were vaccinated intramuscularly with the modified pCC27 DNA vaccine.Then the protective immunity and the humoral immune respo se in pigs were done. The animal test showed that the TGG epitopes could increas e the level of IgG and more importantly, of IgG 2a,IgG 1 pCC27-specific a ntibodies. The higher duration of IgG response induced by the modified DNA vacci ne pVAX-STGG-CC27 was longlived. The protective rate induced in immunized newb orn pigs was 89%.

关 键 词：猪带绦虫 DNA疫苗 辅助性T淋巴细胞表位 谷胱甘肽还原酶 

分 类 号：R392.11[医药卫生—免疫学]