儿童乙型肝炎病毒基本核心启动子变异与血清肿瘤坏死因子的相关性研究  

Relationship between HBV BCP mutation in children with hepatitis B and content of serum tumor necrosis factor-α

作　　者：陈宝昌[1] 高国林[2] 李彦敏[1] 王炳辉[1] 田朝霞[1] 韩小梅[1] 董红红[1] 王娜[1] 

机构地区：[1]河北医科大学第一医院儿科,河北石家庄050031 [2]河北省儿童医院儿内科,河北石家庄050031

出　　处：《临床荟萃》2004年第16期912-914,共3页Clinical Focus

摘　　要：目的　探讨儿童乙型肝炎病毒基本核心启动子 (HBVBCP)变异与肿瘤坏死因子 α(TNF α)水平的相关性及其意义。方法　采用核酸分子杂交技术检测儿童乙型肝炎病毒双位点变异 (T176 2 /A176 4 ) ,同时以酶联免疫吸附测定法检测其血清TNF α水平 ,另外 ,进行了肝功能检测 ,对结果进行统计学分析。结果　儿童慢性乙型肝炎轻、中、重度 3组HBVBCP基因区双变异检出率分别为 9.38%、2 8.5 7%、5 5 .5 6 % ;HBVBCP变异株病毒感染组肝功能损害程度较野生株病毒感染组严重 ,经统计学处理 ,差异有统计学意义 ;HBVBCP变异株病毒感染组血清TNF α水平显著高于非变异株病毒感染组患儿。结论　儿童慢性乙型肝炎HBVBCP双变异株病毒感染后 ,引起TNF α产生增多 ,使肝炎病变程度加重 。Objective To explore the relationship and significance between the two mutations in hepatitis B virus basic core promoter(HBV BCP) gene of children with chronic hepatitis B and the level of serum tumor necrosis factor-α(TNF-α). Methods The two point mutations, T1762/A1764, were probed by polymerase chain reaction-microplate nucleotide acid molecular hybridization, and the content of serum TNF-α was tested by enzyme-linked immunosorbent assay. In addition, concentrations of the serum alanine transaminase(ALT) and serum bilirubin(SB) were also detected. And then the results were analyzed by statistic methods. Results The positive rates of HBV BCP gene mutations from mild, middle and severe groups were 9.38%, 28.57%, and 55.56%, respectively. The levels of serum ALT and SB of the children with HBV BCP gene mutations were obviously higher than those of the other group; the content of TNF-α in positive mutation group was also higher than that of the other group with statistical significance. Conclusion The two point mutations in children with chronic hepatitis B lead to the proliferation of TNF-α, and deteriorate hepatosis. The detection of HBV BCP gene mutations and concent of serum TNF-α may be valuable to prognosis of patients and preventive mechanism of severe hepatitis B.

关 键 词：肝炎病毒 乙型 启动区(遗传学) 突变 肿瘤坏死因子 

分 类 号：R373.2[医药卫生—病原生物学]