糖尿病视网膜病变早期血管内皮生长因子作用机制的研究  

Mechanisms of vascular endothelial growth factor in diabetic rat retinopathy

作　　者：宋鄂[1] 董宇[1] 王浩东[2] 徐琪[3] 王心蕊[4] 吴家祥[4] 

机构地区：[1]吉林大学第一医院眼科,吉林长春130021 [2]吉林大学医疗系,吉林长春130021 [3]吉林大学基础医学部生理学教研室,吉林长春130021 [4]吉林大学基础医学部病理解剖学教研室,吉林长春130021

出　　处：《中国病理生理杂志》2004年第8期1440-1443,共4页Chinese Journal of Pathophysiology

基　　金：吉林省科学基金资助项目 (No .990 5 75 - 5 ) ;吉林大学新技术新疗法基金资助项目 (No.2 0 0 10 9)

摘　　要：目的 :通过链脲佐菌素 (streptozocin ,STZ )糖尿病大鼠视网膜的石蜡切片及血管铺片 ,研究糖尿病大鼠视网膜病变时血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)作用的分子病理机制。方法 :建立糖尿病大鼠模型 ,随机分为正常对照组 (C) ,糖尿病 1月组 (M1)、3月组 (M3 )、5月组 (M5)。分别在视网膜石蜡切片及血管铺片上行VEGF原位杂交和免疫组化。结果 :①石蜡切片 :原位杂交仅M5表达为 6 7% ;免疫组化M3 表达为 34% ,M5表达为 89%。②视网膜血管铺片 :原位杂交仅M5表达为 34% ;免疫组化仅M5表达为 5 6 %。结论 :①除内皮细胞外 ,周细胞及M櫣ｌｌｅｒ细胞也可产生ＶＥＧＦ ;AIM: To study the molecular mechanism of vascular endothelial growth factor (VEGF) in pathogenesis of diabetes in rats. METHODS: The diabetic rat model was established by using streptozocin. The animals were divided into normal control (C group), diabetic for one month (M_1 group), for three months (M_3 group) and for five months (M_5 group). In situ hybridization and immunohistochemistry were conduced to observe the expression of VEGF on retinal digest preparation and paraffin section. RESULTS: 1. On paraffin section: the positive rate of VEGF expression in M_5 group was 67% by in situ hybridization and 89% by immunohistochemistry. There was only 34% positive expression of VEGF in M_3 group by immunohistochemistry. 2. On retinal digest preparation: the VEGF positive expression rate in M_5 group was 34% by in situ hybridization and 56% by immunohistochemistry. CONCLUSION: Both endothelial cells and mural cells and Müller cells express VEGF. The source of VEGF may be from the paracrine pathway in early stage of diabetic retinopathy.

关 键 词：糖尿病视网膜病变 免疫组织化学 原位杂交 内皮生长因子 

分 类 号：R587.1[医药卫生—内分泌]