Benextramine逆转神经肽Y下调人血管平滑肌细胞LDL-R的表达  

Influence of benextramine on the expression of low density lipoprotein receptor in human vascular smooth muscle cells induced by neuropeptide Y

作　　者：梁若斯[1] 谢富康[2] 

机构地区：[1]广州医学院组织胚胎学教研室,广东广州510182 [2]中山大学医学院组织胚胎学教研室,广东广州510080

出　　处：《中国病理生理杂志》2004年第8期1449-1453,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目 (No .980 4 6 6 )

摘　　要：目的 :探讨benextramine对神经肽Y(NPY)人血管平滑肌细胞 (hVSMC)低密度脂蛋白受体 (LDL -R)表达影响的逆转作用。方法 :应用荧光免疫组化和激光扫描共聚焦显微技术 ,定量检测hVSMC的LDL -R表达的变化。Benextramine(B组 )浓度设为 10 -5mol/L ,NPY设 10 -8mol/L(N1组 )、10 -7mol/L(N2组 )、10 -6mol/L(N3组 )和 10 -5mol/L(N4组 ) 4个浓度 ,培养时间分别为 6、12、2 4和 4 8h。结果 :对照组LDL -R表达的平均荧光值较高 ,介于 2 5 6 4 -2 6 4 9之间 ,且各时段间均无显著差异 (P >0 0 5 )。各NPY组LDL -R表达的平均荧光值均明显低于对照组和各benextramine组 (P <0 0 5 ) ,介于 16 39- 2 5 12之间 ,其荧光值随NPY浓度增加而下降 ,N1-N4组分别平均下降15 6 4 %、2 0 31%、2 2 5 8%和 2 7 4 2 % ,呈现一定的剂量依赖效应 ;同时其荧光值也随NPY作用时间的延长而下降 ,在6、12、2 4和 4 8h时间段分别平均下降 11 2 4 %、2 1 2 9%、2 3 0 4 %和 30 38% ,亦呈现一定的时间依赖效应。各benex tramine组 (包括B组、B +N1组、B +N2组、B +N3组及B +N4组 )的LDL -R表达荧光值介于 2 5 5 4 - 2 6 31之间 ,与对照组间差异无显著 (P >0 0 5 ) ,且与NPY作用浓度及时间无关 (P >0 0 5 )。结论 :NPY能导致hVSMC?AIM: To explore the influence of benextramine on the expression of low density lipoprotein receptor (LDL-R) in human vascular smooth muscle cells (hVSMCs) treated with neuropeptide Y (NPY). METHODS: The quantitative fluorescent immunocytochemistry was applied to examine the expression of LDL-R in hVSMCs through laser scanning confocal micropscope system. The concentration of benextramine was 10^(-5) mol/L (B group) and the concentrations of NPY were 10^(-8) mol/L (N1 group), 10^(-7) mol/L (N2 group), 10^(-6) mol/L (N3 group) and 10^(-5) mol/L (N4 group). The culture time was 6, 12, 24 and 48 h, respectively. RESULTS: The average fluorescent value of the LDL-R expression in control group was high (from (2 564) to (2 649)) and there was no significant difference among the different culture time (P>0.05). The average fluorescent value in NPY group was lower than that in control and benextramine groups(form (1 639) to (2 512))(P<0.05), decreasing by 15.64%, 20.31%, 22.58% and 27.42%, respectively in N1-N4 groups in a dose-dependent manner. Besides, the average fluorescent value in N1-N4 groups decreased by 11.24%, 21.29%, 23.04% and 30.38%, respectively at 6, 12, 24 and 48 h in a time-dependent manner. The average fluorescent value of the LDL-R expression in benextramine groups (including B+N1 group, B+N2 group, B+N3 group and B+N4 group) was also high (from (2 554) to (2 631)) and there was no significant difference between benextramine groups and control (P>0.05). The average fluorescent value of benextramine groups were not affected by the action time and the concentrations of NPY(P>0.05). CONCLUSION: NPY could downregulate the expression of LDL-R in hVSMCs and the downregulation was blocked by benextramine.

关 键 词：Benextramine 神经肽Y 肌 平滑 血管 受体 LDL 动脉硬化 人类 

分 类 号：Q253[生物学—细胞生物学]