动脉粥样硬化斑块中细胞凋亡及其相关基因的表达  

Apoptosis and expression of its related genes in human atherosclerotic plaques

作　　者：陈跃峰[1] 阴赪茜[2] 杜心清[2] 邱建龙[3] 刘玳瑄[4] 黄种心[3] 王莉莉[3] 杨建胜[4] 蔡旭东[4] 

机构地区：[1]中国协和医科大学中国医学科学院阜外心血管病医院心血管病研究所冠心病诊疗中心,北京100037 [2]福建医科大学附属第二医院心内科 [3]福建医科大学附属第二医院病理科 [4]福建医科大学附属第二医院心胸外科

出　　处：《临床心血管病杂志》2004年第8期451-454,共4页Journal of Clinical Cardiology

基　　金：福建省卫生厅资助 (No :2 0 0 0 0 6 )

摘　　要：目的 :研究动脉粥样硬化 (AS)病灶中细胞凋亡的发生情况及其相关基因的表达情况 ,探讨细胞凋亡与bcl 2、p5 3、C myc基因表达的内在联系。方法 :选择因AS住院手术患者 2 0例作为研究对象〔其中男 17例 ,女 3例 ,平均年龄 (6 6 .8± 9.86 )岁 ,主要取患者的股动脉〕 ,及因意外伤害手术患者的 10例〔其中男 8例 ,女 2例 ,平均年龄 (5 1.6± 11.97)岁〕作为正常对照。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记 (TUNEL)技术检测两组动脉标本的细胞凋亡情况 ,采用免疫组化的方法检测bcl 2、p5 3及C myc基因的表达情况 ,应用透射电镜进行两组动脉标本的细胞形态学观察。结果 :AS病灶中 ,细胞凋亡的发生率明显高于正常对照血管 (P <0 .0 1) ,bcl 2蛋白的表达明显降低 ,p5 3蛋白及C myc蛋白的表达明显升高 (P <0 .0 1) ;透射电镜证实AS病灶中有大量的平滑肌细胞发生凋亡。结论 :细胞凋亡是AS病灶中细胞死亡的一种主要形式 ,bcl 2、p5 3及C myc基因的表达可能对细胞凋亡的发生起重要的调控作用。Objective:To study apoptosis and expressions of protooncogenes bcl-2, p53 and C-myc of cells in human atherosclerotic (AS) plaques.Method:Arterial specimens were derived from 20 patients operated as arteriosclerosis and 10 victims. Cell apoptosis was in site detected with the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and transmission electron microscope. The expressions of protooncogenes bcl-2, p53 and C-myc was detected with immunohistochemistry.Result:Compared with normal arteries, more cells were found to be positively stained with TUNEL in AS plaques (P< 0.01). Lots of smooth muscle cells in AS plaques were found to be apoptosis with electron microscope. The expression of protooncogene bcl-2 was markedly decreased in AS plaques, but the expressions of p53 and c-myc was markedly increased (P< 0.01).Conclusion:Apoptosis is a main type of cell death in human AS plaques. The expressions of protooncogenes bcl-2, p53 and C-myc may play an important role in regulating the apoptosis.

关 键 词：动脉粥样硬化 细胞凋亡 基因 BCL 2 基因 P53 

分 类 号：R543.5[医药卫生—心血管疾病]