菟丝子活性成分槲皮素含量测定中HPLC方法的建立  

Establishment of HPLC Method in Assaying Quercetin in Dodder Seed

作　　者：曾诚[1] 罗颂平[1] 宓穗卿[2] 黄天来[2] 李坤寅[1] 骆运晖[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510407 [2]广州中医药大学临床药理研究所,广东广州510405

出　　处：《中国中医药信息杂志》2004年第8期701-703,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的 建立菟丝子中槲皮素的含量测定方法。方法 采用高效液相色谱-紫外检测法(HPLC-UV),以卡马西平为内标,菟丝子灌胃给药后,大鼠含药血清样品经液-液萃取后在C18反相色谱柱上进行分离测定。结果 槲皮素血药浓度在0.05～9 μg/mL范围内线性关系良好(r2=0.9996)。方法回收率为86.65%～99.07%,提取回收率为79.91%～84.56%,最低检测限为0.05 μg/mL。结论 本方法稳定可靠,结果可为菟丝子在临床用药的安全性和有效性提供依据。Objective To find the method of assaying quercetin level in dodder seed. Methods The quick and sensitive HPLC-UV method was established with carbamazepine acting as internal standard. After the rats were douched with dodder seed, drug-containing serum samples of rats were separated and measured on C18 reverse-phase chromatography column after being liquid to liquid extracted. Results The linear range of calibration curve is 0.05~9 靏/mL (r2=0.9996). The method showed good precision and recoveries for serum with 86.65%~99.07%, extracting recoveries 79.91%~84.56%, and the limit of detection (LOD) is 0.05 靏/mL. Conclusion The method is stable and reliable, and the results can provide scientific basis of safety and effectiveness for clinical use of dodder seed.

关 键 词：菟丝子 槲皮素 卡马西平 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]