新制抗癌方对AFB_1染毒的HBV转基因小鼠肝癌前病变组织PCNA、P21^(ras)和NF-KBP65表达的影响  

Effects of New Anti-tumor Formular on Expression of PCNA,P21 ^(ras) and NF-KB P65 in Liver Precancerous Lesions of HBV Large Envelope Transgenic Mice Injected by Aflatoxin B1

作　　者：朱梅菊[1] 张红[2] 郭振球[3] 

机构地区：[1]湛江师范学院体育系运动医学教研室,广东湛江524048 [2]湖南中医学院附一院肿瘤科,湖南长沙410007 [3]湖南中医学院中医诊断研究所,湖南长沙410007

出　　处：《中国中医基础医学杂志》2004年第7期58-61,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：广东省教育厅自然科学基金资助项目 ( 2 0 0 0 49);广东省湛江市科委 988科技兴湛项目

摘　　要：Objectives:This study is to investigate the effects of new anti-tumor formular(NAF)on expression of PCNA,P21 ras and NF-KB P65 in liver precancerous lesions of HBV large envelope transgenic mice injected by aflatoxin B1(AFB1).Methods:The precancerous HBV large envelope transgenic mouse injected by AFB1 liver model was used.Mice was given water,NAF concentrated water solution throughout the whole experiment(48 weeks).PCNA,P21 ras and NF-KB P65 protein expression were detected by immunohistochemical method.Results: PCNA,P21 ras and NF-KB P65 expressions were significantly inhibited by NAF treatment.Conclusion NAF inhibited PCNA,P21 ras and NF-KB P65 protein expressions,therefore,NAF could show obvious effects on protecting against synergistic hepatoearcinogenesis of HBV and AFB1.Objectives:This study is to investigate the effects of new anti-tumor formular(NAF)on expression of PCNA,P21 ras and NF-KB P65 in liver precancerous lesions of HBV large envelope transgenic mice injected by aflatoxin B1(AFB1).Methods:The precancerous HBV large envelope transgenic mouse injected by AFB1 liver model was used.Mice was given water,NAF concentrated water solution throughout the whole experiment(48 weeks).PCNA,P21 ras and NF-KB P65 protein expression were detected by immunohistochemical method.Results: PCNA,P21 ras and NF-KB P65 expressions were significantly inhibited by NAF treatment.Conclusion NAF inhibited PCNA,P21 ras and NF-KB P65 protein expressions,therefore,NAF could show obvious effects on protecting against synergistic hepatoearcinogenesis of HBV and AFB1.

关 键 词：transgenic mice PRECANCEROUS conditions liver neoplasms PCNA P21^RAS NF-KB P65 

分 类 号：R285.5[医药卫生—中药学]