小鼠触须毛囊外根鞘细胞的体外培养及uPA和uPAR表达研究  

Culture of outer root sheath cells of murine vibrissa follicles in vitro and expression of uPA/uPAR

作　　者：高强国[1] 符刚[1] 杨恬[1] 

机构地区：[1]第三军医大学基础医学部细胞生物学教研室,重庆400038

出　　处：《第三军医大学学报》2004年第16期1452-1454,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 2 70 6 71)~~

摘　　要：目的　建立小鼠毛囊外根鞘细胞的体外培养方法及研究尿激酶型纤溶酶原激活剂及其受体 (uPA uPAR)的表达。方法　建立以毛囊真皮鞘细胞为滋养层 ,培养外根鞘细胞并对uPA uPAR的表达进行免疫细胞化学鉴定。结果　外根鞘细胞在此滋养层上生长较好 ,并有uPA uPAR的表达。结论　毛囊真皮鞘细胞是外根鞘细胞较好的滋养层 。Objective To establish a method for the cultivation of hain follicle outer root sheath (ORS) cells of murine vibrissa and to study their expression of uPA/uPAR. Methods The ORS cells of murine vibrissa were cultured by combination of enzyme digestion and tissue culture with the feeder layer of dermal sheath cells of the hair follicle. The cells and their uPA/uPAR expression were identified by immunocytochemistry (ICC). Results The feeder layer of DS was suitable for the culture of ORS cells. ORS cells expressed uPA/uPAR protein. Conclusion The DS of hair follicle is a better feeder layer for the growth of ORS cells. ORS cells have the ability to migrate and proliferate.

关 键 词：毛囊 外根鞘细胞 真皮鞘细胞 UPA/UPAR 

分 类 号：R322.995[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]