兔肢体缓慢牵伸延长对相邻关节软骨代谢的影响  

Effects of limb lengthening on metabolism of articular cartilage in experimental rabbits

作　　者：王序全[1] 李起鸿[1] 许建中[1] 周强[1] 

机构地区：[1]第三军医大学西南医院骨科,重庆400038

出　　处：《第三军医大学学报》2004年第15期1368-1370,共3页Journal of Third Military Medical University

摘　　要：目的　探讨肢体缓慢牵伸刺激作用下相邻关节软骨的功能代谢变化及其生物适应能力。方法　 3 0只健康成年新西兰兔分为延长组 (A)及延迟观察组 (B) ,再按延长幅度不同分为 10 %(A1 0 ,B1 0 )、2 0 %(A2 0 ,B2 0 )及 40 %(A4 0 ,B4 0 ) 3亚组 ,每组 5只 ,另外 5只作正常对照。采用胫骨上干骺端截骨缓慢牵伸肢体延长 ,延长速度 1mm d ,分上、下午 2次完成。定期摄X线片监测延长幅度及膝关节影像学变化。生物化学方法测定预定延长幅度时的胫骨平台关节软骨基质糖胺聚糖 (glycosaminoglycan ,GAG)含量及羟赖氨酸与羟脯氨酸构成比变化。结果　①各组不同时相点膝关节软骨未见明显影像学异常。②A1 0 、B1 0 及B2 0 组软骨GAG含量正常 ;A2 0 组仅氨基半乳糖显著性下降 (P <0 .0 5 ) ;A4 0 组中氨基己糖、氨基半乳糖和葡萄糖醛酸均出现明显下降 (P <0 .0 1)而氨基葡萄糖并无明显变化 (P >0 .0 5 ) ;B4 0 组GAG含量较A4 0 组均有显著性恢复(P <0 .0 1) ,其中氨基己糖及氨基半乳糖已恢复至正常水平。③A4 0 及B4 0 组软骨基质胶原中HYL HYP比分别为 0 18及 0 .17,其余各组之比值接近于正常比 0 .2 4左右。结论　低幅度延长时关节软骨代谢功能能有效适应缓慢牵伸刺激 。Objective To investigate the adaptation of metabolism of the arthrodial cartilage for limb lengthening. Methods Thirty New Zealand rabbits were divided into two groups: limb lengthening group (A) and delayed observation group (B). Each group was further divided into three sub groups ( n =10) according to the distraction ranges: 10%, 20%, and 40%. Upper tibia metaphysis lengthening was adopted at 1 mm/d rate of distraction and iconography changes of the knee joint were observed with X ray. The contents of glycosaminoglycan (GAG), hydroxylysine (HYL), and hydroxyproline (HYP) were determined by biochemical techniques. Results ① There was no obvious imaging change of knee articular cartilage at every time point in each group. ② The content of GAG was normal in group A 10 , and B 10 , and B 20 , but the content of galactosamine decreased obviously in group A 20 ( P <0.05). In group A 40 , the contents of hexosamine, galactosamine, and glycuronic acid were obviously decreased ( P <0.01), but hexosamine and galactosamine recovered to the normal level ( P >0.05). The content of GAG recovered obviously in group B 40 as compared with that in group A 40 . ③ The ratio of HYL to HYP was 0.18 and 0.17, respectively in group A 40 and group B 40 , but it was normal in other groups, nearly 0.24. Conclusion Metabolism of the arthrodial cartilage during low distraction range can adapt the stimulation of distraction, but great distraction range may result in transitory disorder in metabolic function of the arthrodial cartilage.

关 键 词：肢体延长 关节软骨 代谢 

分 类 号：R322.72[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]