基于cDNA宏阵列的系统聚类分析猪发育阶段的基因表达谱  

Cluster Analysis of the Gene Expression Patterns Based on cDNA Macroarray in Different Development Stages of Fetal Skeletal Muscles in Swine

作　　者：朱正茂[1] 赵书红[1] 陈浩[2] 余梅[1] 刘榜[1] 樊斌[1] 朱猛进[1] 李奎[1] 

机构地区：[1]华中农业大学动物科技学院,湖北武汉430070 [2]华中农业大学理学院,湖北武汉430070

出　　处：《武汉大学学报（理学版）》2004年第4期482-486,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30100131);国家863计划项目(2001AA222241);国家重大基础研究和发展计划项目(G2000016103);国家杰出青年基金资助项目(39925027)

摘　　要：取杜洛克猪胚胎第33,45,55,65,75天的背最长肌样本,用cDNAMacroarray分析方法和聚类分析技术分析了327个EST在骨骼肌内不同发育阶段的基因表达谱,结果表明有98条EST在不同发育时期显著差异表达.第33天和第45天两阶段基因表达状态相似,第55天和第65天基因表达状态相似,而第75天的基因表达与第55天和第65天两个阶段的基因表达具有相近的聚类关系.表达状态相近,基因功能相似的基因大都被聚类在一起.To examine the application of cDNA macroarray in pork production trait candidate genes discovery, cluster analysis for the expression data was tested among the five development stages: 33 days, 45 d, 55 d, 65 d and 75 d of gestation. The results indicated that development stage with similar gene expression action mode were clustered together. 33 d and 45 d were clustered together;also 55 d and 65 d were clustered together based on their expression patterns.75 d,which was known the stage that the number of muscle fibers began to stop increasing,had a close clustering position with 55 and 65 d group.The genes that had the similar function or joined the same process were most likely clustered together. And 98 ESTs differently expressed among the five development stages were indicated by using linear mixed models analysis.

关 键 词：CDNA MACROARRAY 猪 胚胎 骨骼肌 发育 聚类分析 

分 类 号：Q953[生物学—动物学]