鞘内注射6-羟多巴胺、α_1受体拮抗剂对氯胺酮脊髓镇痛作用的影响  

Effecet of intrathecal injection of 6-OHDA or α_1-adrenoceptor antagonist on the spinal analgesia of ketamine

作　　者：葛志军[1] 戴体俊[1] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002

出　　处：《中国药理学通报》2004年第8期864-867,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目;No 3 9970 715;江苏省自然科学基金资助项目;BK2 0 0 114 3

摘　　要：目的　在体探讨脊髓去甲肾上腺素 (NE)能神经元α1受体和氯胺酮 (Ket)脊髓镇痛的关系。方法　用热水甩尾法观察鞘内注射 (ith)氯胺酮 5 0、10 0、2 0 0 μg对小鼠甩尾潜伏期(TFL)的影响。并观察鞘内分别预先注射 6 羟多巴胺 (6 OH DA ,6 μg)、α1受体拮抗剂哌唑嗪 (Pra,5、15 μg)或特拉唑嗪(Ter,5、15 μg )对Ket(10 0 μg ,ith)脊髓镇痛的影响。 结果　Ket(5 0 μg ,ith)对小鼠TFL无影响 (P >0 0 5 ) ,而Ket(10 0、2 0 0μg ,ith)可剂量依赖性地延长小鼠TFL(P <0 0 5 )。鞘内单独注射 6 OHDA、Pra或Ter对小鼠的痛阈无影响 (P >0 0 5 )。ith 5 μgPra或Ter对Ket脊髓镇痛无影响 (P >0 0 5 ) ,而ith 6 OHDA、15 μgPra或Ter则可明显减弱Ket脊髓镇痛 (P <0 0 5 )。结论　脊髓是Ket的镇痛部位之一 。Aim To investigate the relationship between spinal cord noradrenergic neurons α 1 adrenoceptors and the spinal analgesia of ketamine. Methods Kunming mice were used. Analgesia tests were investigated with warm water tail flick test. The effects of intrathecal injection (ith) of ketamine (50,100,200 μg)on tail flick latency of animals were observed. And the effect of pretreatment with intrathecal 6 hydrodoapa(6 OHDA, 6μg ) and α 1 adrenoceptor antagonist prazosin (5, 15 μg) or terazosin (5, 15 μg) , respectively on the spinal analgesia of ketamine (100 μg,ith) was studied. Results Dose dependent analgesia was observed following ith ketamine (100,200 μg, P <0 05). Intrathecal 6 OHDA, prazosin or terzosin alone did not affect basal tail flick latency. Pretreatment with intrathecal 5 μg prazosin or terzosin had no effect on the spinal analgesia of ketamine, but pretreatment with intrathecal 6 OHDA , 15 μg prazosin or terazosin significantly reduced the spinal analgesia of ith ketamine but not 5 μg ( P <0 05). Conclusion Spinal cord is one of the targets of ketamine analgesia. The spinal analgesia of ketamine is partly mediated by spinal noradrenregic neurons and α 1 adrenoceptors.

关 键 词：氯胺酮 脊髓镇痛 去甲肾上腺素能神经元 Α1受体 6-OHDA 哌唑嗪 特拉唑嗪 

分 类 号：R332[医药卫生—人体生理学] R332.81[医药卫生—基础医学]