西洋参茎叶皂苷对CPHD患者细胞免疫功能的影响  

Effect of PQS on the cell immune system of the patients with CPHD

作　　者：许力军[1] 段秀梅[1] 钱东华[1] 于保东[1] 孔凡玉[1] 刘克辉[2] 

机构地区：[1]吉林大学第一医院呼吸科,吉林长春130021 [2]吉林大学第一医院内分泌科,吉林长春130021

出　　处：《中国药理学通报》2004年第8期901-903,共3页Chinese Pharmacological Bulletin

基　　金：吉林省科技厅自然科学基金资助项目(2 0 0 10 5 3 0 )

摘　　要：目的　通过观察西洋参茎叶皂苷对慢性肺原性心脏病 (CPHD)患者细胞免疫功能的影响 ,探讨CPHD患者的发病与机体免疫的关系。方法　采用流式细胞术检测 2 0例CPHD患者外周血T淋巴细胞亚群和NK(CD3-/CD16 +5 6 + )细胞 ;RT PCR方法检测IL 2、IFNγmRNA的表达。结果　CPHD患者CD3+ 、CD4 + 、和CD4 /CD8比值明显低于对照组并差异有显著性 ,而CD8+ 细胞高于对照组 (P <0 0 1)。PQS治疗后CD3+ 、CD4 + 、CD3-/CD16 +5 6 + 细胞和CD4 /CD8比值都升高 ,其中CD4 + 、CD3+ 细胞与治疗前比较呈显著性差异 (P <0 0 5 ) ,而CD8+ 细胞与治疗前比较降低 (P <0 0 5 )。西洋参茎叶皂苷 (PQS)能促进T细胞分泌细胞因子IL 2、IFNγmRNA的表达。结论　当CPHD患者免疫功能低下时 。Aim To investigate the effect of Panax Quinquefolium Saponin (PQS) on the cell immune system of the patients with Chronic Pulmonary Heart Disease (CPHD) and then to find out the relationship between the immune system and the mechanism of CPHD. Methods The T lymph subgroup and NK (CD3 -/CD16+56 +) cells in patients’ peripheral blood were detected by flow cytometry, and the expression of IL 2, IFNγ mRNA were analyzed by RT PCR. Results CD3 +, CD4 + and CD4/CD8 in patients with CPHD were significantly lower than those in the control, while CD8 + higher ( P <0 01). CD3 +, CD4 +, CD4/CD8 and NK (CD3 -/CD16+56 +) cells in the patients were increased after PQS treated, and CD3 +, CD4 + were significantly different from pre treated ( P <0 05), while CD8 + cells were significantly lower than those in pre treated ( P <0 05). PQS stimulated the expression of IL 2, IFNγ mRNA excreted by T lymph cells. Conclusions The results showed that PQS can improve the cell immune function, especially in patients with CPHD whose immune function are low.

关 键 词：慢性肺原性心脏病 西洋参茎叶皂苷 免疫功能 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]