用质粒Gap-LCR-ELISA法检测孕妇尿中沙眼衣原体  

The substances inhibiting plasmid Gap-LCR-ELISA test for detecting Chlamydia trachomatis in the urine of pregnant women

作　　者：伍金林[1] 韦红[1] 吴仕孝[1] 钟晓云[1] 余加林[1] 

机构地区：[1]重庆医科大学附属儿童医院儿科研究所,重庆400014

出　　处：《临床皮肤科杂志》2004年第9期523-525,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30200300)

摘　　要：目的:研究孕妇尿液中对质粒缺口-连接酶链反应(Gap-LCR)-ELISA法检测沙眼衣原体(CT)产生抑制作用的物质及其去除方法,以提高该方法检测无症状孕妇CT感染的灵敏度。方法:对181份孕妇首段尿(FVU)进行全面尿化学分析后,采用CT标准株“Spike”法(铆钉法),对出现Gap—LCR—ELISA抑制的标本用多因素非条件Logistic回归分析与抑制作用相关的成分,在进行一系列去抑制处理后比较各方法的转阳率。结果:孕妇FVU对质粒Gap-LCR-ELISA检测CT的抑制率为6.08％(11/181),其中白细胞和磷酸盐结晶与抑制作用相关;对FVU作-70℃～37℃快速冻融2次的预处理可使90.91％的假阴性转为阳性,并对模板无明显降解作用。结论:孕妇尿液中确有对Gap—LCR—ELISA产生抑制作用的物质存在,可通过改良标本预处理的方法降低其所致的假阴性。Objective: To study the substances inhibiting plasmid Gap-LCR-ELISA test of detecting Chlcanydia trachomatis (CT) in the urine of pregnant women. Methods: One hundred and eighty one FVU were subjected to comprehensive urinalysis including dipstick and microscopic examinations. CT standard strain was diluted with the FVU and detected using the plasmid Gap-LCR-ELISA test. The results were analyzed by logistic regression analysis to single out suspicious factors associated with inhibition. Results: 6.08% of the urine samples were negative in plasmid Gap-LCR-ELISA test. The logistic regression analysis showed that leukocytes as well as phosphate crystal were attributed to the inhibitory effect in the urine. After 2 time pre-treating of FVU fast freezing-thawing at -70-37℃, 90.91% false negative urine samples reversed to the positive. Conclusions: The leukocytes and phosphate crystal can inhibit plasmid Gap-LCR-ELISA test for CT detecting in the urine of pregnant women. These inhibitors could be removed by adequate disposal-method of the FVU.

关 键 词：沙眼衣原体 尿 孕妇 质粒缺口-连接酶链反应-酶联免疫吸附法 抑制物 

分 类 号：R374.1[医药卫生—病原生物学]