AT_1R基因多态性与原发性高血压合并脑血管病的相关性研究  

Correlations of AT_1R Gene Polymorphism to Cerebrovascular Diseases in Essential Hypertensive Patients

作　　者：石静萍[1] 张颖冬[1] 许利刚[1] 葛剑青[1] 钱敏[1] 

机构地区：[1]南京医科大学附属南京脑科医院神经内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2004年第5期487-490,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省科技厅自然科学基金(应用基础)资助项目(BJ98092)

摘　　要：目的探讨Ⅰ型血管紧张素Ⅱ受体(AT1R)基因多态性对原发性高血压人群脑血管病发生的影响。方法应用多聚酶链反应-限制性片段长度多态性(PCR鄄RFLP)方法检测60例正常对照者、45例原发性高血压无合并症(EH)、33例原发性高血压合并脑出血(EH鄄CH)及73例原发性高血压合并脑梗死(EH鄄CI)患者AT1R基因3'非翻译区A1166C变异的多态性。结果①EH组和EH鄄CH组的AC+CC基因型频率与对照组比较差异有显著性(均P<0.05),两组的C等位基因频率(分别为0.29和0.33)显著性高于对照组(0.17),但两组间基因型频率和C等位基因频率比较无显著性差异;②EH鄄CI组的基因型频率分布及C等位基因频率与对照组、EH组比较均差异无显著性;③逐步Logistic回归表明AT1R基因多态性是EH发生的独立危险因素(OR=0.44,P<0.05),但未证实AT1R基因多态性是EH鄄CH发病的独立危险因素;④AT1R基因多态性与高血压合并脑血管病患者的收缩压和舒张压水平呈正相关(分别r=0.23和0.25,均P<0.05),而与糖、脂代谢无相关。结论AT1R基因1166C等位基因可能通过对血压的影响而间接参与高血压人群脑出血的发病,但与高血压患者是否发生脑梗死可能无关。Objective: To investigate the association of angiotensinⅡ typeⅠreceptor (AT1R) gene polymorphism with cerebrovascu-lar diseases in essential hypertensive patients. Methods: Forty-five essential hypertensive patients without complication of cerebrovascular diseases(EH), 33 EH patients with cerebral hemorrhage(EH-CH), 73 EH patients with cerebral infarction(EH-CI), 60 healthy controls were genotyped for the A1166C variation at the 3′-untranslated region of AT1R gene by PCR/DdeⅠrestriction endonuclease digestion. Results:① For A1166C locus of AT1R gene, distributions of genotypes frequencies of EH and EH-CH group were significantly different from healthy controls(P < 0.05). The frequencies of C in EH and EH-CH group were significantly higher than those in healthy controls(0.29 vs 0.17, P < 0.05; 0.33 vs 0.17, P < 0.01 respectively), but there was no significant difference in the frequencies of genotypes and C allele between EH and EH-CH group. ② No significant differences were found in the frequencies of genotypes and C allele as comparing EH, healthy controls with EH-CI group. ③Logistic regression analysis showed the polymorphism of AT1R gene was one of the independent risk factors for EH(P < 0.05, OR = 0.44), but not for EH-CH.④The correlative analysis showed a positive correlation between the polymorphisms of AT1R gene and systolic blood pressure as well as diastolic blood pressure in patients with essential hypertension complicated with cerebrovascular diseases(r= 0.23 and 0.25 respectiveres, P < 0.05). Conclusion: By increasing blood pressure, the A1166C allele of AT1R gene may contribute to the occurrence of EH-CH.

关 键 词：血管紧张素Ⅱ受体 基因 原发性高血压 脑血管病 

分 类 号：R541.3[医药卫生—心血管疾病] R743[医药卫生—内科学]