萝卜细胞质青花菜雄性不育系花药发育的细胞形态学研究  

STUDY ON CYTOMORPHOLOGY OF ANTHER DEVELOPMENT IN MALE STERILE LINE OF BROCCOLIC (Brassica oleracea L.var. italica Plenck) WITH RADISH CYTOPLASM

作　　者：朱玉英[1] 龚静[1] 吴晓光[1] 金海军[1] 姚文岳[1] 李式军[2] 吴琴生[2] 

机构地区：[1]上海市农业科学院园艺研究所,上海201106 [2]南京农业大学园艺学院,南京210095

出　　处：《上海农业学报》2004年第3期42-44,共3页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目 [农科攻字 ( 96)第 5 -0 2号 ]

摘　　要：以萝卜细胞质青花菜 (BrassicaoleraceaL .var .italicaPlenck)雄性不育系及其保持系为试验材料 ,选择不同发育阶段的花蕾 ,取其花药 ,制成石蜡切片和超薄切片 ,经染色 ,分别在光学和电子显微镜下观察。结果表明 ,青花菜细胞质雄性不育系与保持系的花药发育有明显的不同 :不育系花药发育受阻于花粉母细胞分化期 ,不能形成正常的四分体小孢子 ,细胞组织逐步解体 ,形成空腔花药 ;保持系花粉母细胞能形成正常的四分体 ,进而形成小孢子 ,最终形成充满正常花粉粒的花药。This paper reports the microstructure of microsporogenesis of male sterile line,BC7-19 in broccoli with radish cytoplasm by making wax slices and ultra-slices of anthers at different growth stage. The results showed that the anther development of male sterility line was obviously different from that of maintainer line. The anthers of male sterile line was obstructed from developing in the differentiation period of pollen mother cell(PMC) and the normal tetrads could not form, Its cell tissue disintegrated gradually and finally the anther finally became hollow. While the PMC of maintainer line could develop smoothly, the normal tetrads could form, then microspores, and finally the pollens could form.

关 键 词：青花菜 萝卜细胞质雄性不育系 花药发育 细胞形态学 小孢子 四分体 

分 类 号：S635.9[农业科学—蔬菜学]