睾酮定向诱导MSC分化的实验研究  

Experimental Study on the Oriented Differentiation of Mesenchymal Stem Cells with Testosterone

作　　者：董晓先[1] 刘金保[1] 董伟华[1] 赵汉宁[1] 郑晴雯[1] 

机构地区：[1]广州医学院病理生理学教研室,广东广州510182

出　　处：《广州医学院学报》2004年第2期1-3,共3页Academic Journal of Guangzhou Medical College

基　　金：国家863计划基金(No.2002AA204011);广州市科技攻关引导项目(No.2003Z3-E0012)

摘　　要：目的:激素体外定向诱导大鼠骨髓间质干细胞(MSC)分化为神经元样细胞。方法:通过贴壁法分离大鼠MSC,体外扩增培养,睾酮定向诱导MSC分化为类神经元样细胞。光镜下观察细胞形态,免疫细胞化学检测神经细胞特异性抗原标志。结果:大鼠骨髓间质干细胞可通过贴壁法成功分离并可在体外大量扩增。睾酮诱导6h后大部分MSC转变为神经元样细胞,出现胞体和突起,免疫细胞化学染色NSE、Nestin呈阳性、GFAP阴性。结论:大鼠骨髓间质干细胞可在体外经睾酮诱导分化为神经元样细胞。Objective :To investigate the effect of testosterone in in-vitro oriented induction of the differentiation of rat mesenchymal stem cells into neuron-like cells. Methods: Mesenchymal stem cells were separated from bone marrow of rats by wall sticking method, amplifying cultured in vitro, and differentiated into neuron-like cells by oriented induction with testosterone. The morphology of cells was observed under light microscopy,. Neuro-specific enolase (NSE), nestin and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry. Results: Rat mesenchymal stem cells could be separated and amplified in vitro. After being induced by testosterone for 6 hrs, most of the cells would be differentiated into neuron-like cells. By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP. Conclusion: Rat mesenchymal stem cells could be induced to differentiate into neuronlike cells with testosterone.

关 键 词：骨髓间质干细胞 神经细胞 睾酮 定向分化 

分 类 号：Q254[生物学—细胞生物学]