毛细管电泳法测定大熊猫尿肌酐  

DETERMINATION OF CREATININE IN URINE OF GIANT PANDAS BY CAPILLARY ELECTRIPHORESIS

作　　者：田红[1] 陈媛[1] 黄晨西[1] 孙儒泳[1] 刘定震[1] 

机构地区：[1]北京师范大学生命科学学院生物多样性与生态工程教育部重点实验室,北京100875

出　　处：《北京师范大学学报（自然科学版）》2004年第4期531-533,共3页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目 (30 170 16 9)

摘　　要：采用毛细管电泳技术测定大熊猫尿液中的肌酐含量 .使用非涂层毛细管 (2 1cm× 75 μmi.d .) ,5 0mmol·L- 1pH2 .5 6的磷酸缓冲液 ,在 16kV ,2 5℃下 ,以吡啶做内标物对大熊猫尿样进行检测 .肌酐标准曲线的质量浓度范围为 5～ 80mg·L- 1,肌酐浓度与肌酐同吡啶的峰高比值的线性相关系数r=0 .9996 .测定了 18只成年大熊猫 (N(♀ )∶N(♂ ) =5∶4 )的 12 2个尿样 ,雌、雄大熊猫的尿肌酐质量浓度分别为 (84 2 .35± 116 .4 8)和 (6 99.92± 90 .94 )mg·L- 1.实验表明 ,毛细管电泳法可以快捷。The determination of creatinine in Giant panda urine by capillary electrophoresis(CE) is described. With a 21 cm×75 μm fused-silica capillary, these samples are achieved using pyridine as internal standard, which is run in a phosphoric acid buffer (50 mmol·L -1, pH2.56) at 25 ℃ with a separation voltage of 16 kV and detected by UV-detector at 200 nm wavelength. The standard curve of creatinine ranges from 5 mg·L -1 to 80 mg·L -1, and the correlation coefficient between the concentrations of creatinine and the corresponding peak height ratios of creatinine and pyridine is 0.999 6. 122 urine samples from 18 adult Giant pandas (N(♀)∶N(♂)=5∶4) are determined and the content of creatinine is (842.35±116.48) mg·L -1 in female Giant panda urines and (699.92±90.94) mg·L -1 in male urines, respectively. The results indicate that CE is rapid and accurate to determine the content of creatinine in the urine.

关 键 词：毛细管电泳 肌酐 大熊猫 尿 

分 类 号：Q959.838[生物学—动物学] Q592.2