纳米SiO_2与常规SiO_2粉尘对大鼠肺脏器系数和羟脯氨酸含量影响的比较研究  

Comparative Study of Lung/Body Coefficient and Hydroxyproline in Rat Lung Exposed to Nano-silica and Normal Silica

作　　者：董静[1] 谢妮[1] 陈莹[1] 金一和[1] 陈杰[1] 

机构地区：[1]中国医科大学公共卫生学院,辽宁沈阳110001

出　　处：《工业卫生与职业病》2004年第5期262-264,共3页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金资助项目 ( 3 0 2 7113 5 )

摘　　要：目的　比较纳米SiO2 与常规SiO2 粉尘对大鼠的肺脏脏器系数和羟脯氨酸含量的影响。方法　分别用粒径为 (1 0± 5 )nm的纳米级SiO2 和粒径为 0 5～ 1 0 μm的常规SiO2 粉尘对雌性Wistar大鼠肺脏染尘 ,每鼠 2 0mg,于染尘后第 30天和第 6 0天测定动物肺脏脏器系数和羟脯氨酸含量。结果　染尘后第 30天、第 6 0天纳米SiO2 组大鼠肺脏脏器系数 [(8 36± 2 4 4 ) ,(7 2 2± 0 92 ) ]和羟脯氨酸含量 [(2 4 5± 0 39) ,(2 70± 0 4 8)mg/g]均低于常规SiO2 组 ,差异有显著性 (P <0 0 5或P <0 0 1 ) ,而与对照组相比差异未见显著性 (P >0 0 5 )。染尘后第 6 0天纳米SiO2 组大鼠全肺湿重 [(2 0 0± 0 2 3) g]低于常规SiO2 组 [(2 4 7±0 6 3) g],差异有显著性 (P <0 0 5 )。 结论　纳米SiO2 较常规SiO2 粉尘致肺脏脏器系数和羟脯氨酸含量增加的程度轻。Objective To comparatively study the lung/body coefficient and hydroxyproline in rat lung exposed to nano silica and normal silica.Methods Female Wistar rats were exposed to lower dose( 20 mg/rat ) of nano SiO 2 and normal SiO 2 by intratracheal injection.Measure rats lung/body coefficient and lung hydroxyproline on 30 d and 60 d .Results The lung/body coefficient and hydroxyproline in nano SiO 2 groups were obviously lower than those of normal SiO 2 groups(P<0 05,P<0 01)on 30 d and 60 d .The wet weights of whole rat lung in nano SiO 2[(2 00±0 23)g]groups were lower than those of normal SiO 2 groups[(2 47±0 63)g,P<0 05] on 60 d .Conclusions The increase of lung/body coefficient and lung hydroxyproline was less in SiO 2 nanoparticles than that of normal SiO 2.

关 键 词：纳米二氧化硅 常规二氧化硅 羟脯氨酸 肺脏器系数 

分 类 号：R135.2[医药卫生—劳动卫生]