丹酚酸B对缺氧损伤心脏微血管内皮细胞细胞间粘附分子表达的影响  

Effects of salveanolic acid B on expression of intercellular adhesion molecule-1 on cardiac microvascular endothelial cells of hypoxia

作　　者：杜嵘[1] 张伯礼[1] 高秀梅[1] 郭利平[2] 张萌[1] 刘洋[1] 商洪才[1] 王虹[1] 

机构地区：[1]天津中医学院,天津300193 [2]天津中医学院第一附属医院,天津300193

出　　处：《中国新药与临床杂志》2004年第9期616-618,共3页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家重点基础研究发展规划项目(G19990 5 4 4 0 3)

摘　　要：目的 :探讨丹参有效成分丹酚酸B在心脏微血管内皮细胞 (CMEC)缺氧 /复氧损伤中的保护作用。方法 :通过心脏微血管内皮细胞体外培养技术 ,建立缺氧预适应 (HPC)模型并给予丹酚酸B ,应用酶联免疫方法检测细胞间粘附分子 1(ICAM 1)表达。结果 :在CMEC缺氧 3h ,复氧 6h后ICAM 1表达升高 ;缺氧预适应和应用丹酚酸B对CMEC进行预处理则能有效降低CMECICAM 1的表达。结论 :丹酚酸B在CMEC缺氧 /复氧损伤中可产生与缺氧预适应类似的保护效果。AIM: To investigate the protective effect of salveanolic acid B (Sal B) on cardiac microvascular endothelial cells (CMEC) injury after hypoxia and reoxygenation. METHODS: By the technique of cultivating CMEC in vitro, the damaging model of hypoxia preconditioning was established and Sal B was added. The expression of ICAM-1 was detected by ELISA. RESULTS: The expression of ICAM-1 of CMEC after hypoxia and reoxygenation is increased. The expression of ICAM-1 on CMEC could be reduced by hypoxia preconditioning and application of Sal B. CONCLUSION: The Sal B preconditioning can bring about the protective effect to CMEC injury after hypoxia and reoxygenation as same as that of ischemic preconditioning.

关 键 词：细胞低氧 细胞粘附分子 内皮 血管 心肌再灌注损伤 丹参 丹酚酸B 

分 类 号：R285.5[医药卫生—中药学] R972[医药卫生—中医学]