高频振动对尾部悬吊大鼠比目鱼肌梭内、外肌纤维肌球蛋白重链表达的影响  

Effects of High Frequency Vibration on Expression of Myosin Heavy Chain(MHC) in Intrafusal and Extrafusal Fibers in Soleus Muscles of Tail-suspended Rats.

作　　者：杨威[1] 樊小力[1] 吴苏娣[1] 宋新爱[1] 

机构地区：[1]西安交通大学医学院生理学教研室,陕西西安710061

出　　处：《航天医学与医学工程》2004年第3期166-170,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助 ( 3 0 0 70 83 0 )

摘　　要：目的观察高频正弦波振动对尾部悬吊大鼠比目鱼肌 (SOL)梭内、外肌纤维MHC表达的影响。方法采用大鼠尾部悬吊法建立模拟失重模型 ,高频正弦波振动大鼠比目鱼肌。用免疫组织化学技术 ,观察大鼠比目鱼肌 (SOL)梭内、外肌纤维MHC表达变化。结果尾部悬吊 7d后大鼠比目鱼肌梭内、外肌纤维中快缩型MHC的表达均有所增加 ,而在吊尾期间给予高频正弦波振动比目鱼肌后梭内、外肌快缩型MHC表达没有明显变化。结论高频正弦波振动对尾部悬吊大鼠比目鱼肌梭内、外肌纤维MHC表型转化有明显的对抗作用。Objective To study the effects of high frequency vibration on expression of myosin heavy chain(MHC) in intrafusal and extrafusal fibers in soleus muscles of tail-suspended rats. Method Weightlessness was simulated by tail suspension of female rats.Using immunohistochemistry technique,changes of expression of MHC in intrafusal and extrafusal fibers of soleus muscles were detected. Result Expression of fast MHC in intrafusal and extrafusal fibers of soleus muscles increased during 7 d of simulated weightlessness,whereas during 7 d of tail suspenion plus high frequency vibration,these changes were not detected.Conclusion High frequency vibration can counteract the changes in expression of MHC in intrafusal and extrafusal fibers of rats soleus under muscles of rats in simulated weightlessness situation.

关 键 词：失重模拟 尾部悬吊 高频振动 肌梭 基因表达 梭内肌纤维 梭外肌纤维 比目鱼肌 

分 类 号：R854[医药卫生—航空、航天与航海医学] R852.22[医药卫生—临床医学]