聚乳酸纳米微粒的制备及其在鼠脑组织中的选择性分布研究  

PREPARATION AND SELECTIVELY DISTRIBUTION OF POLYLACTIC ACID NANOPARTICLES IN MURINE BRAIN

作　　者：王华芳[1] 胡豫[1] 孙望强[2] 谢长生[2] 魏文宁[1] 周薇[1] 方峻[1] 

机构地区：[1]华中科技大学同济医学院血液病研究所,武汉430022 [2]华中科技大学材料科学与工程学院

出　　处：《中国生物医学工程学报》2004年第4期311-315,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30170392)

摘　　要：制备聚乳酸(polylactic acid, PLA)纳米微粒(以下简称PLA纳粒),研究该载体在小鼠脑组织中的分布特征,探讨其对鼠血脑屏障的透过能力。纳米沉积法制备空白及经聚山梨醇80(T-80)表面修饰的PLA纳粒,透射电镜观测其微观形貌、粒径,zeta电位/粒度分析仪分别测定粒度分布范围和zeta电位值;荧光分光光度计检测PLA纳粒能否进入小鼠脑部;透射电镜观察脑组织中纳粒的分布区域。所制PLA纳粒为规整、均匀的球形颗粒, 其等效粒径为162.1nm, 多分散系数为0.108;T-80修饰的PLA纳粒能特异性地分布于小鼠脑组织,电镜结果显示脑微血管内皮细胞中有纳粒的聚集,神经元间隙也有少量分布。结论:表面修饰的PLA纳粒能较特异性地被鼠脑血管内皮细胞摄取,并能够通过血脑屏障。该载体可望成为选择性作用于脑组织的优良给药载体。The polylactic acid (PLA) nanoparticles, were prepare and the distribution and permeability to the blood-brain barrier (BBB) in murine brain of this vector were studied as well. The PLA nanoparticles with un-stained surface and with Tween-80 modified surface were prepared by the nanoprecipitation method. The morphology and size of PLA nanoparticles were observed by transmission electron microscope (TEM). The polydispersity index and the zeta potential of PLA nanoparticles were measured by a zetasizer. Fluorescence microscope was used to observe brain distribution after the FITC-labelling PLA nanoparticles were i.v. injected to mice. Then the ultrastructure of areas containing nanoparticles in mice was observed by TEM. The produced PLA nanoparticles are uniform spherical particles with an effective diameter of 162.1nm and a polydispersity index of 0.108. The Tween-80 modified PLA nanoparticles can locate selectively in brain, and TEM images showed there were some collections of PLA nanoparticles in brain microvascular endothelial cells (BMECs). A part of nanoparticles dispersed in interspace of neurons. Surface modified PLA nanoparticles can be uptaken specificially by BMECs and have possibility to overcome the BBB. Therefore, the PLA nanoparticles mag be a kind of good biomacromolecule-loaded system with brain targeting.

关 键 词：聚乳酸 纳米微粒 载体 脑组织 分布 

分 类 号：R318.08[医药卫生—生物医学工程]