^(99m)Tc标记结肠癌单克隆抗体片段CEA-Fab的研制方法  

Labeling of Monoclonal Antibody CEA Fab Segment Against Colon Cancer with ^(99m)Tc

作　　者：吕中伟[1] 蔡海东[1] 娄继权[1] 邱桂林[1] 黄飚[2] 顾伟中[1] 

机构地区：[1]同济大学附属第十人民医院核医学科,上海200072 [2]江苏省原子医学研究所,江苏无锡214063

出　　处：《同济大学学报（医学版）》2004年第4期343-344,348,共3页Journal of Tongji University(Medical Science)

摘　　要：目的　探讨一种高效便捷的99mTc CEA Fab标记化合物的研制方法。方法　采用 2 亚氨噻酚盐酸盐修饰CEA Fab ,通过GH转换 ,将99mTc标记在结肠癌单克隆抗体CEA Fab上。结果　已修饰抗体的标记率为 93.5 %,99mTc胶体含量为 2 .5 %,标记化合物有良好的稳定性和免疫活性。结论　成功地建立了结肠癌单抗片断CEA Fab的99mTc标记方法 ,该技术可以应用于其他抗体片段标记领域。Objective To provide an accurate and convenient labeling method of 99m Tc CEA Fab for radionuclide imaging of colon cancer.Methods The monoclonal antibody CEA Fab segment was modified by iminothiolane and the ligand was exchanged from 99m Tc O(GH) - 2.Labeling efficiency was measured by paper chromatography.Results The labeling efficiency of the labeled antibody CEA Fab was 93.5%,with 2.5% colloidal 99m Tc in it.The high stability and immunocompetence of 99m Tc CEA Fab was shown by the competition test in vitro.Conclusion The labeling method of 99m Tc labeled colon cancer monoclonal antibody CEA Fab segment was established successfully.The method appears equally applicable to other labeling antibodies.

关 键 词：癌胚抗原 单克隆抗体 ‰Tc:标记 

分 类 号：R817.4[医药卫生—影像医学与核医学]