云南番茄地方主栽品种及野生种的遗传多样性研究  

The Genetic Diversity of Yunnan Local Varieties and Wild Species of Tomato

作　　者：朱海山[1] 张宏[2] 毛昆明[2] 李洪涛[2] 汪安云 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]云南农业大学园林园艺学院,云南昆明650201 [3]云南省烟草研究院,云南昆明650200

出　　处：《云南农业大学学报》2004年第4期373-377,共5页Journal of Yunnan Agricultural University

基　　金：云南省"十五"攻关项目(2001NG21)

摘　　要：用RAPD技术检测了27份番茄材料的遗传多样性,并对其进行了聚类分析。结果表明,番茄各栽培品种之间的遗传背景十分狭窄,在使用的11条随机引物对番茄基因组DNA进行的RAPD检测中,共产生125条DNA谱带,其中9条带为特征带,14条为27个品种(其中1个为野生种)的共显带,多态带为86条,多态率为68 8%.供试品种被划分为6大类群,其分类与形态学上的分类基本一致。Using RAPD technique and cluster analysis 27 Yunnan local varieties and wild species of tomato were tested for their genetic diversity. The results showed: the genetic background of tomato varieties was narrow based on 11 arbitrary 10-mer oligonucleotide primers with 125 amplification DNA-bands, among which, 86 (68.8%) were polymorphic, 9 were specific, 14 were consensus bands among 27 varieties. All the varieties and wild species were divided into 6 groups based on the DNA band pattern, showing similarity to morphologic classification.

关 键 词：云南 番茄 地方品种 野生种 遗传多样性 

分 类 号：S641.2[农业科学—蔬菜学]