软骨藻酸对小鼠脑组织HO-1蛋白表达的影响  

Effects of domoic acid on heme oxygenase-1 protein expression in mouse brain

作　　者：王斌[1] 李龙[1] 董杰[1] 陈丹[1] 

机构地区：[1]华中科技大学同济医学院卫生毒理学系,武汉430030

出　　处：《中国公共卫生》2004年第1期57-58,共2页Chinese Journal of Public Health

基　　金：湖北省自然科学基金资助 ( 2 0 0 2AB1 2 3)

摘　　要：目的　研究软骨藻酸 (domoicacid)对小鼠脑组织丙二醛 (MDA)含量及血红素氧化酶 -1(hemeoxygenase-1,HO -1)蛋白表达的影响。方法　小鼠连续 5d腹腔注射软骨藻酸染毒后 ,采用硫代巴比妥酸 (TBA )法测定小鼠脑组织中MDA含量 ,并用免疫组织化学方法检测小鼠脑组织中HO -1分布及表达。结果　低剂量 (1/ 90LD5 0 )组海马、高剂量 (1/ 10LD5 0 )组大脑皮层和海马MDA含量明显升高 ,与对照组相比有显著性差异 (P <0 0 5)。对照组大脑皮层和海马HO -1阳性细胞平均光度 (A)分别为 0 0 953± 0 0 2 52和 0 0 940± 0 0 3 0 4;低剂量组皮层和海马A分别为 0 14 10± 0 0 40 7和 0 14 0 6± 0 0 3 54,与对照组相比 ,有显著性差异 (P <0 0 5) ;高剂量组大脑皮层和海马A分别为 0 162 6± 0 0 3 74和 0 160 4± 0 0 3 2 5,与对照组相比 ,有显著性差异 (P <0 0 1)。结论　软骨藻酸对小鼠大脑皮层和海马具有氧化损害作用 ,并诱导皮层和海马HOObjectiveTo study the effects of domic acid on MDA conte nt and heme oxygenase-1(HO-1) protein expression in mouse brain.MethodsTBA method and immunity histochemis try staining were applied to assess the MDA content and HO-1 protein expression respectively in mouse brain after administration domoic acid,ip for 5 days.ResultsThe MDA content in hippocampus in low dose group (1/90 LD\-\{50\})and in high dose group (1/10 LD\-\{50\}) were significantly dif ferent from that in hippocampus in control group ( P <0 05).The MDA content i n cortex of high dose group was significantly different form that in cortex in c ontrol group ( P <0 05).The mean light activity of positive HO-1 cells respec tively was 0 141?0±0 040?7 in hippocampus and 0 140?6±0 035?4 in corte x in low dose group,respectively higher than 0 095?3±0 025?2 in hippocampus and 0 094?0±0 030?4 in cortex in control group ( P <0 05).The mean ligh t activity of positive HO-1 cells respectively was 0 162?6±0 037?4 in hipp ocampus and 0 160?4±0 032?5 in cortex in high dose group,respectively highe r than that in hippocampus and in cortex in control group ( P <0 01).ConclusionDomoic acid could cause oxidative injury in hi ppocampus and cortex in mouse brain and increase HO-1 protein expression as well .

关 键 词：软骨藻酸 氧化损害 血红素氧化酶-1 

分 类 号：R992[医药卫生—毒理学]