L-精氨酸对热应激大鼠胸腺和脾脏的影响  

Effects of supplementary L-arginine on thymus and spleen in heat-stressed rats

作　　者：张云山[1] 罗海吉[2] 吉雁鸿[2] 卢晓翠[2] 

机构地区：[1]海军总医院超声诊断科,北京100037 [2]第一军医大学军队卫生学教研室

出　　处：《中国公共卫生》2004年第5期596-597,共2页Chinese Journal of Public Health

基　　金：军队"九五"课题基金 (96L - 0 2 6)

摘　　要：目的　探讨热应激对大鼠体重、胸腺指数、脾脏指数、呼吸、肛温的影响和补充L -精氨酸 (L -Arg)对热应激大鼠的保护作用。方法　采用三因素析因设计模型 ,将青春期SD雄性大鼠随机分为A、B、C3组 ,通过灌胃补充不同浓度的L -Arg ,观察在常温和不同高温条件下热应激时大鼠胸腺指数等相关指标的变化。 结果　在常温状态下 ,补充L -Arg组大鼠体重、胸腺指数、脾脏指数增加高于未补充组 (P <0 0 5 ) ,呼吸和肛温变化无显著性差异 (P >0 0 5 ) ;而在不同温度热应激状态下 ,各组大鼠体重、胸腺重量、脾脏重量均减轻 ,未补充L -Arg组低于补充L -Arg组 ,并且胸腺和脾脏重量减轻具有温度和热应激后时间依赖性 ;呼吸和肛温在一定范围内随温度的升高而增快和升高 ,但补充与未补充L -Arg组之间无显著性差异 (P >0 0 5 )。结论　补充适量的L -Arg可使大鼠在常温下体重、胸腺指数和脾脏指数有所增加 ,在热应激条件下 。Objective To inquire into the effects of heat stress on the body weight,thymus indexes,spleen indexes,respiration,rectal temperature and the protection function of supplementary L-Arginine(L-Arg) on heat-stressed rats.Methods According to the factorial experiment design for three factors,hebetic male Sprague Dawley rats were randomly divided into A,B,C groups.The relevant indexes change such as the thymus index were observed in SD rats which were exposed under normal temperature and different hyperthermia with supplied different concentration L-Arginine by gavage.Results In normal temperature,the body weight,thymus and spleen indexes increase of the groups supplemented with L-Arginine were higher than those of the control groups gavaged with water( P <0.05),but the changes of respiration and rectal temperature had no significant difference( P >0.05).In the different high temperature heat-stressed condition,the body weight,thymus weight and spleen weight of the heat-stressed rats all decreased in both supplementation of L-Arginine and no supplementation of L-Arginine groups, but these decresses were less in rats with supplementation of L-Arginine groups than those with no supplementation of L-Arginine groups.And the thymus weight and spleen weight of heat stress groups were temperature and time of heat stress dependent.The respiration and rectal temperature followed the increase of temperatare were accelerated and elevated within limits,but there was no significant difference between the groups supplemented with L-Arginine and group without supplemented with L-Arginine( P >0.05).Conclusion Supplied suitable dose of L-Arginine can induce the increase of the body weight,thymus index and spleen index in normal temperature and relieve the atrophy of the thymus and spleen in heat stress.

关 键 词：L-精氨酸(L-Arg) 热应激 体重 胸腺指数 脾脏指数 

分 类 号：R122.21[医药卫生—环境卫生学] R994.7[医药卫生—公共卫生与预防医学]